摘要:
应用猪链球菌2型定型PCR方法,对从福建省3个屠宰场采集的临床健康的生猪鼻咽拭子样本共121份进行了检测,同时对阳性样本进行了细菌分离鉴定及毒力基因检测。结果显示:121份样本中有17份为阳性,检出率为14.0%(17/121),其中福州地区检出率为10.9%(7/64),宁德地区检出率为11.8%(4/34),莆田地区检出率为26.1%(6/23)。3株分离菌cps2J基因PCR特异性扩增产物大小均为675 bp,其核苷酸序列与Genebank中猪链球菌2型参考菌株的同源性高达98%～100%,毒力基因型为gdh+/cps2J+/mrp+/ef-/sly-/fbps+/orf2+。调查结果表明,福建省福州、宁德、莆田等地区生猪群中均存在猪链球菌2型携带者,所分离到的3个猪链球菌2型菌株其毒力基因型为新的基因型。
Abstract:
One-hundred-twenty-one nasopharyngeal swab samples of slaughtered,clinically healthy pigs from 3 slaughter houses in different areas of Fujian were collected for isolation of Streptococcus suis type 2(SS2) and detection of the virulence genotype with specific PCR.The results showed a total positive rate of SS2 of 14.0%(17/121) among the pigs sampled.The positive rates in Fuzhou,Ningde and Putian were 10.9%(7/64),11.8%(4/34) and 26.1%(6/23),respectively.The PCR amplified products of the cps2J gene from 3 isolates were all 675bp in size. The cps2J gene nucleotide homology between the isolates and the SS2 strain,as published in Genbank,ranged from 98%-100%.The virulence genotypes of the 3 isolates were all gdh+/cps2J+/mrp+/ef-/sly-/fbps+/orf2+. The results suggested that the SS2 existed in the clinically healthy pigs in the selected areas of Fujian.The virulence genotype of the SS2 isolated in this study was not previously known.
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