Abstract: The genetic polymorphism of 5’-regulatory region of IGF-1 gene in Dehua black chicken was analyzed by PCR-PstⅠ-RFLP.The relationship between the polymorphic locus and egg-laying was established using the least square analysis.There were three genotypes,AA,AB and BB,detected on the PstⅠsite.The result showed that there was no significant difference in the age of the chickens when egg-laying started,but there was a significant difference in the number of eggs laid at 240 days and 300 days(P0.05).The numbers of eggs at 240 days and 300 days for the chickens with the AA genotype was significantly higher than those with the AB or BB(P0.05). The number of eggs laid at 240 days and 300 days for the chickens with the AB genotype was higher than that with BB,but not significantly(P0.05).