利用TAC系统构建多个光合同化物运输蛋白基因转化载体和水稻的遗传转化

Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice

摘要: 采用人工染色体(TAC)多基因组装载体系统,将一组与水稻光合同化物运输转化有关基因构建在同一载体上,这些基因分别是蔗糖转运蛋白基因(OsSUT5),单糖转运蛋白(OsMST4)、细胞壁转化酶(OsCIN3)和ATP/ADP转运蛋白(OAAT),获得了包含这4个基因的植物表达载体 pTAC4H-OAAT-SUT5-CIN3-MST4,质粒大小近30 kb.通过农杆菌转化法,将基因导入水稻,以探讨这些基因对水稻光合同化物运输和转化的调控作用.

Abstract: By using a transformation-competent artificial chromosome vector pYLTAC747H,we constructed four genes relating to assimilation translocation,including OsSUT5,OsMST4,OsCIN3 and OAAT in the expression vector,pTAC747H-OAAT-SUT5-CIN3-MST4.The entire inserted fragment was approximately 30 kb.By the agrobacterium-mediated transformation,7 independent clones were obtained.PCR detection showed that 4 target genes and one marker gene had been integrated into the rice genome.