谷物酿造料酒非生物浑浊的快速微量测定

陈嘉西; 关天庆; 李勇强; 万培耀; 封弘渊; 徐岩; 王栋

谷物酿造料酒非生物浑浊的快速微量测定

Rapid Abiotic Turbidity Determination of Grain-brewed Cooking Wine

摘要

摘要:
目的针对谷物酿造料酒行业长久以来存在的非生物浑浊研究中缺乏明确的浑浊度测定方法及其应用标准的问题，建立一种适合的测定方法，实现谷物酿造料酒非生物浑浊的快速微量测定及澄清判定。
方法基于吸光度检测原理，在不同波长下测定样品的吸光度OD值以及样品与其膜过滤清液的吸光度差值ΔOD，通过与浊度仪测定的样品浊度值进行相关性分析，确定较为准确的表征料酒浑浊度的方法，并对谷物酿造料酒样品进行检测和统计分析。
结果确定了以450 nm下测得的吸光度差值ΔOD₄₅₀表征谷物酿造料酒浑浊度的方法，该方法测定值与浊度仪测定的浊度值具有良好的相关性（R²=0.936）。在此基础上，提出了ΔOD₄₅₀≤0.024作为谷物酿造料酒澄清的客观判别标准，判定准确率达到90％以上。
结论该方法样品用量少，操作简便，可同时测定多个样品，能较准确地测定谷物酿造料酒的浑浊度，进而可对料酒样品是否澄清进行评判，为料酒行业的品质控制提供依据。

Abstract:
Objective A rapid method to measure the abiotic turbidity of grain-brewed cooking wine was established.
Method Based on light absorbance of fluids, optical density (OD) at varied wavelengths of light measured by a spectrometer on membrane-filtered supernatant of grain-brewed cooking wine were obtained with absorbance difference (ΔOD) calculated. The data were correlated with turbidity readings (NTU) determined by a turbidimeter on same samples.
Result A method to rapidly and accurately measure the turbidity of a grain-brewed cooking wine applying ΔOD at the wavelength of 450nm was established. The measurements significantly correlated with the NTUs obtained by the turbidimeter (R²=0.936). ΔOD₄₅₀ of 0.024 was set as the threshold for acceptable clarity of cooking wines, with an accuracy rate of greater than 90%.
Conclusion The newly developed method required small sample size, simple operation, and short testing time. It could be applied for quality control for the industry.

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