Abstract: The abolone seedlings at shell length of(16.3±0.2)mm,density of 1633/m2 have been cultivated in 6 pools at the condition of 19.6-26℃,salinity 30,and the effect on abalone seedling culture between two modes have been compared,the water-saving mode of adding microbiological agents and the traditional mode.After 60 days of culture,the shell length,survival rate and average daily gain of the abalone seedlings between the test group and the control group were(21.57± 0.40)mm and(23.17±0.38)mm,(93.69±0.46)% and(91.61±1.55)%,(87.77±4.21)μmd-1 and(114.42±6.76)μmd-1.The result showed that: after adding micro-biological agents,75.76% of cultivation water was saved,the effect was significant(P0.05),and it can help reduce the adverse effects of water exchange,and improve the survival rate of abalone seedling cultivation(P0.05).