Abstract: With the application of Jun-Cao technique,the Chamaecrista Rotundifolia with the capacity of improving soil fertility and water conservation in red soil mountain area,local mountain grass Miscanthus and straw were used to build heap fermentation and cultivate Agaricus Blazei under counter-ecological cultivation.And the comparative analysis of yield and fatty acids,amino acid and polysaccharide content of fruiting body to Agaricus Blazei can provide a new technique mode based on counter-ecological cultivation of wild Agaricus Blazei and eco-cycle cultivation under Gualou shacks.