木霉菌及其代谢产物对香蕉枯萎病菌的离体抑制作用研究

In vitro study on inhibition of Trichoderma sp. and their metabolites against Fusarium oxysporum f. sp. cubense

摘要: 离体测定了22株木霉菌及其代谢产物对香蕉枯萎病菌4号生理小种的抑制作用,结果表明采用平板对峙培养,木霉菌对香蕉枯萎病原菌菌丝生长的抑制率为61.76%~100.00%,20%(V/V)木霉菌发酵液对病原菌菌丝生长抑制率为11.83%~52.69%,而木霉菌产生的非挥发性代谢产物和挥发性代谢产物对病原菌菌丝生长抑制率分别为3.83%~74.62%和11.17%~29.37%。用20%~50%(V/V)木霉菌T05-049发酵液处理香蕉枯萎病菌球型分生孢子9 h和镰刀型分生孢子6 h,其孢子萌发抑制率分别为86.60%~96.40%和56.29%~85.41%。

Abstract: Inhibition effect of 22 strains of Trichoderma sp.and their metabolites against Fusarium oxysporum f.sp.cubense race four(FOC)was studied in vitro.The bioassay data obtained indicated that the inhibition rates of(a) the tested strains on the FOC mycelia growth in the plate-culture experiments ranged from 61.76% to 100%,(b) the 20%(V/V) T.sp.culture filtrates were 11.83%-52.69%,(c) the non-volatile and volatile metabolites were 3.83%-74.62% and 11.17%-29.37%,respectively,and(d) the spherical conidia spore germination from FOC treated with 20%-50%(V/V) of T.05-049 culture filtrate for 9h and the sickle-shaped conidia for 6h were 86.60%-96.40% and 56.29%-85.41%,respectively.