反硝化细菌的筛选及其亚硝酸盐降解特性研究

Isolation and nitrite degradation ability of a denitrifying bacterium

摘要: 从海水养殖池的底泥中筛选到6株反硝化细菌,其中对亚硝酸盐降解力最强的1株菌JN1初步鉴定为假单胞菌,研究了JN1的亚硝酸盐降解特性,结果表明:JN1具有高效降解转化亚硝酸钠的能力,其适宜降解条件为:亚硝酸钠浓度1 000~4 000 mgL-1,菌种接种量5%(v/v),培养基装量100 mL(250 mL容量),中量充气发酵,发酵时间18~30 h,发酵pH值6.5~7.5。

Abstract: Six strains of denitrifying bacteria were isolated from the mud in marine aquaculture ponds.Among them,one showed the strongest nitrite degradation capability.The strain was subsequently identified to be a Pseudomonas sp.and named JN1.Its optimum nitrite degradation conditions were determined in the lab to be at 1 000-4 000 mgL-1 sodium nitrite concentration,5%(v/v) bacterial inoculums and pH 6.5-7.5 with 100 mL of fill volume in a 250 mL flask and moderate aeration in the fermentation for 18-30 h.