Abstract:
Objective Bacteria antagonistic to Ganoderma multipileum that causes root rot on Delonix regia were identified, and their culture conditions optimized for biocontrol of the increasingly spread disease on the landscape ornamental trees.
Method Using dual culture method, anti-G. multipileum microbes were isolated with taxonomic identities determined by morphological and molecular means. Conditions for culturing the isolates were optimized. In vitro control efficacy on the pathogen was examined by a pot experiment.
Result The consortium of Strains FH-3 and FH-7, which were identified as Pseudomonas aeruginosa and Bacillus licheniformis, respectively, exhibited an inhibition rate of 72.80% against G. multipileum. In single-factor and orthogonal experiments, the culture of the mixed consortium was optimized to have a medium containing 3% of sucrose, 0.5% of ammonium sulfate, 1% of beef extract, and 2% of potassium chloride with 3% of inoculum to propagate under 32 ℃, pH 7, and constant stirring at 220 r·min−1 for 24 h. As a result, the inhibition rate on G. multipileum was raised to 88.70%.
Conclusion The mixed FH-3 and FH-7 significantly inhibited the growth of G. multipileum, indicating a potential of developing the strains for controlling the root rot disease on D. regia.
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