Abstract: Adding tea-branch dust as substitution in Ganoderma lucidum cultivation, its effects on the biomass and the content of Ganoderma polysaccharide and triterpenes were analyzed. The results showed that, by using tea-branch dust in culture, Ganoderma lucidum grew well with normal fruits obtained and spores released. Comparing with control group, the Ganoderma lucidum in substitution group decreased the biological efficiency by 6.25%-31.33%, increased the content of polysaccharide and triterpenes 3.03%-28.28% and 2.62%-17.40%, respectively. The results suggest that tea-branch dust can be used as a substitution in Ganoderma lucidum cultivation but the application amount should be not more than 70%.