Abstract: Free amino acids in the flowers of 30 loquat germplasms were determined using an automatic amino acid analyzer. The results showed that all samples contained 16 amino acids. Among them, 7 were essential amino acids, including Phe, Thr and Lys. The content of Thr was the highest (i.e., 238.46-727.45 mghg-1) and Met the lowest (i.e., 0.21-3.92 mghg-1). The greatest contents of TAA, EAA and NAA were found in the flowers of Dayeyangdun loquat, which were 2.69, 2.95 and 3.11 times of the lowest contents, respectively. No significant differences were found among the 30 loquat germplasms in the contents of TAA and NAA, nor EAA/TAA ratio. But, EAA in the flowers of loquat germplasms from different regions showed significant differences. No significant differences were found on the contents of TAA, EAA and NAA or EAA/TAA ratio in the flowers of loquat germplasms with flesh of different colors. The average NAA in flowers of several wild loquat germplasms was significantly lower than that of the loquat cultivars, while TAA and EAA showed no significant differences.