青枯雷尔氏菌致病性生物测定方法的研究

车建美; 刘波; 张彦; 苏明星; 朱育菁; 郑雪芳

青枯雷尔氏菌致病性生物测定方法的研究

福建省农业科学院农业生物资源研究所,福建福州 350003

基金项目:

国家自然科学基金项目(30871667);福建省自然科学基金项目(2008J0054);福建省财政专项福建省农业科学院科技创新团队建设项目(STIF-Y03)

详细信息

作者简介:
车建美(1977-),女,博士,副研究员,研究方向:生物技术及生物防治(E-mail:chejm2002@163.com)

通讯作者:
刘波(1957-),男,博士,研究员,主要从事微生物生物技术与农业生物农药研究(E-mail:liubofaas@163.com)

中图分类号: S 436
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出版历程
- 收稿日期: 2011-08-09
- 修回日期: 2011-09-04
- 刊出日期: 2011-10-14

Study on the Bioassay Method for Pathogenicity of Ralstonia solanacearum

Agricultural Bio-resource Research Institute,Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 采用不同浓度的强致病力青枯雷尔氏菌FJAT-91处理番茄盆栽苗,建立青枯雷尔氏菌致病性生物测定方法。结合菌落形态和特异性引物鉴定结果说明所分离得到的菌株均为强致病力青枯雷尔氏菌。当番茄植株培养至第5 d时,4个不同接种浓度处理的番茄植株苗均出现发病症状。采用104 cfumL-1处理的番茄植株在第4 d发病,但到第8 d,发病率也达到90%左右。随着青枯雷尔氏菌接种浓度的递增,各处理番茄植株死亡率达到致死中浓度(LC50)的时间逐渐缩短。104 cfumL-1的接种浓度效果最佳, 既缩短试验周期,又保证了番茄青枯病的发生。
- 青枯雷尔氏菌 /
- 强致病力 /
- 生物测定 /
- 番茄
Abstract: The bioassay method of virulent Ralstonia solanacearum strain was established on the tomato plants treated by different concentration of virulent R. solanacearum strain FJAT-91. The results showed that the R. solanacearum strains isolated from wilted tomato plants were virulent strains according to the colony form and test of polymerase chain reaction (PCR) with special primers. The disease symptom of tomato plants treated with different concentrations appeared on the fifth day. The tomato plants began wilting on the fourth day, which were treated with concentration of 104 cfumL-1, and the incidence was reached 90% after 8 days. Time to LC50 of tomato plants was shortened along with the increasing of concentration of R.solanacearum. Therefore, the concentration of 104 cfumL-1 is the optimal one that could shorten test period and make the tomato plant wilt.
- Ralstonia solanacearum /
- virulent /
- bioassay /
- tomato