应用RAPD标记鉴定苦瓜杂种纯度

RAPD Determination on Purity of Hybrid Bitter Gourd Seeds

摘要: 以苦瓜杂交种闽研2号及其亲本为材料,利用RAPD标记技术对苦瓜杂交种的多态性进行分析。结果表明:在供试的200条RAPD引物中,筛选出1个引物S115能区分杂交种及其亲本种子。利用互补型RAPD引物S115对苦瓜杂交种闽研2号的纯度进行了快速鉴定,结果与田间鉴定结果一致。

Abstract: Polymorphisms of the hybrid bitter gourd, Minyan 2, and its parents lines were analyzed by using RAPD markers.Selected from 200 RAPD primers, the specific primer, S115, was found to be capable of differentiating the hybrid Minyan 2 from its parents.Genetic purity of Minyan 2 was further tested with S115 to agree with the field test results.