Screening Biological Control Agents, Bacillus spp., Against Fusarium oxyspomm f sp. Cubense and its Inhibitory Characteristics
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摘要: 从我国不同地区采集土样,共分离到300余株芽胞杆菌,通过平板对峙生长法从中筛选到4株对香蕉枯萎病原菌有明显拮抗作用的菌株。经16S rRNA 序列分析,将这4株生防芽胞杆菌鉴定为多粘类芽胞杆菌。4株菌的生物学特性基本一致,其主要脂肪酸类型为15∶0 ANTEISO,分析发现生防菌对病原菌的抑菌作用与生防菌的脂肪酸类型具有相关性。从菌量、温度、pH以及紫外照射时间对4株生防菌的抑菌特性进行了研究,结果表明:4株菌的抑菌效果随着浓度的降低而降低,当菌液稀释100倍时只有FJAT-4539具有较强的抑菌作用;4株菌的抑菌作用对温度敏感,加热会导致抑菌活性丧失;4株菌均在pH为9时对病原菌具有强的抑菌作用;4株菌对紫外耐受力强。综合所有试验结果,菌株FJAT-4539对香蕉枯萎病的抑菌效果最强。Abstract: More than three hundred Bacillus spp.were isolated from the soil samples in China, and there were four strains that had effective in suppression Fusarium oxyspomm f sp.cubense on PDA agar plates.These four strains were identified as Paenibacillus polymyxa based on 16S rRNA sequence.There were similar biological characterics, and main fatty acid type was 15∶0 ANTEISO among those strains.There are correlation between the inhibitory effect and some fatty acid based on DPS soft analysis.The characterization, such as different account, temperature, pH and UV time, were studied,and the effect of 4 Paenibacillus polymyxa against Fusarium oxyspomm f sp.cubense were also studied in this study.The results showed that the effect of four Bacillus species were decreased by concentration, and sensitive to temperature.And four strains had strong inhibitory effect at pH9, and not sensitive to UV.The results showed that that strain FJAT-4539 was the strongest strain to inhibit banana wilt disease among the four Bacillus spp.
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张晓舟, 徐剑宏, 李顺鹏. 植病生防芽胞杆菌的分离筛选与初步鉴定[J].土壤. 2005, 37(1):85-88. TIMMUSK S,NICANDER B,GRANHALL U,et al. Cytokinin production by Paenibacillus polymyxa [J].Soil Biology and Biochemistry, 1999, 31:1 847-1 852.
DIJKSTERHUIS J, SANDERS M, GOMS LG, et al.Antibiosis play a role in the context of direct interaction during an tagonism of Paenibacillus polymyxa towards Fusarium oxyspomm [J]. Journal of Applied Microbiology, 1999, 86:13-21.
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