Abstract: More than three hundred Bacillus spp.were isolated from the soil samples in China, and there were four strains that had effective in suppression Fusarium oxyspomm f sp.cubense on PDA agar plates.These four strains were identified as Paenibacillus polymyxa based on 16S rRNA sequence.There were similar biological characterics, and main fatty acid type was 15∶0 ANTEISO among those strains.There are correlation between the inhibitory effect and some fatty acid based on DPS soft analysis.The characterization, such as different account, temperature, pH and UV time, were studied,and the effect of 4 Paenibacillus polymyxa against Fusarium oxyspomm f sp.cubense were also studied in this study.The results showed that the effect of four Bacillus species were decreased by concentration, and sensitive to temperature.And four strains had strong inhibitory effect at pH9, and not sensitive to UV.The results showed that that strain FJAT-4539 was the strongest strain to inhibit banana wilt disease among the four Bacillus spp.