菌株LPF-1(Brevibacillus brevis LPF-1)降解猪粪过程中降解物质的GS-MS分析
Detection of Hog Manure Degradation by Brevibacillus brevis LPF-1 with GC-MS
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摘要: 利用气-质联用(GS-MS)分析猪粪污降解菌LPF-1(Brevibacillus brevis LPF-1)降解猪粪过程中挥发性物质的变化。以匹配度大于80(最大为100),含量大于5%为标准,在3个处理中共检测到各类物质23种,其中菌株LPF-1发酵液检测到的物质种类最多,有16种;猪粪+水溶液处理检测到12种,其中苯类物质3种(1,2-二甲苯、对二甲苯、间二甲苯),酚类物质4种(2,4-二叔丁基苯酚、苯酚、2,5-二叔丁基酚、4-甲基苯酚);猪粪+LPF-1发酵液处理检测到物质10种,其中苯类物质1种(对二甲苯),酚类物质3种(2,4-二叔丁基苯酚、苯酚、4-甲基苯酚),菌株LPF-1能有效减少猪粪降解过程中苯类和酚类等有害物质种类的产生,具有进一步开发成粪污降解微生物菌剂潜力。Abstract: Effect of Brevibacillus brevis LPF-1 on volatile organic compounds(VOCs)in hog manure degradation were studied with Gas Chromatography/Mass Spectrometry(GS-MS).There were twelty-three VOCs in three samples under the condition of matching degree80 and relative content5%.A total of sixteen VOCs were detected in liquid fermentation of strain LPF-1, as compared to twelve VOCs in mix liquid of hog manure with three chlorobenzens and four phenolics and ten VOCs in mix liquid of hog manure and strain LPF-1 with a chlorobenzens and three phenolics.The results showed that sorts of chlorobenzens and phenolics could be reduced effectively by strain LPF-1 in hog manure decomposition.
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