Abstract: Meiotic behaviors of pollen mother cells (PMCs) of bitter gourd (Momordica charantia L.) were characterized. It was found that the pollen mother cell of bitter gourd revealed a simultaneous cytokinesis with unsynchronized phenomenon in meiotic division. Nucleolus disappeared gradually from prophase I to metaphase I, reappeared at prophaseⅡand then disappeared. Chromosome became more and more identifiable from prophase I to metaphase I, and illegible at telophase I with unwinding. Moreover, multi nucleoli was investigated in prophase I and prophase Ⅱ. Frequent polyads were observed in this study, which was likely related to the weather adversity and unsynchronized phenomenon in meiotic division.