香蕉种质资源亲缘关系的ISSR分析

姚锦爱; 蔡鸿娇; 石妞妞; 甘林; 杨秀娟; 陈福如

香蕉种质资源亲缘关系的ISSR分析

1.
福建省农业科学院植物保护研究所,福建福州 350013;
2.
集美大学水产学院,福建厦门 361021

基金项目:

福建省自然科学基金项目(2011J01114);福建省发改委高新技术项目(2011-352);农业科技成果转化资金项目(2009GB2C400174);福建省科技创新平台建设项目(2009N2005)

详细信息

通讯作者:
杨秀娟(1972-),女,硕士,副研究员,主要从事植物病理学研究

中图分类号: S 668.1
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出版历程
- 收稿日期: 2011-09-19
- 修回日期: 2011-11-09
- 刊出日期: 2012-01-29

Genetic Relationship among Banana (Musa spp.) Germplasms Revealed by ISSR Analysis

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Fisheries College of Jimei University, Xiamen, Fujian 361021, China

摘要

摘要: 利用ISSR标记对3个基因型(AAA,AA 和 ABB)共35份香蕉种质资源的遗传多样性和亲缘关系进行了分析,筛选出的6条ISSR引物共扩增出38条带,扩增片段长度为200~2 000 bp,27条具有DNA多态性,多态性为71.05%,遗传距离为4.30%~33.33%。用UPGMA聚类分析结果显示,以遗传距离25%为阈值,35份香蕉种质可分成6个类群,除贡蕉(AA)和金粉1号(ABB)分别归为Ⅲ和Ⅳ两个类群之外,其余33份种质分为4个类群,其中Ⅰ类群包含着来自不同地方的基因型为AAA和ABB的22份香蕉种质。基因型为AAA和ABB的香蕉种质并不被简单地划分成两类,部分基因型相同的香蕉种质之间没有表现出紧密的亲缘关系。本研究所用的6条引物能把香蕉种质区分开,ISSR分子标记对研究香蕉种质资源的亲缘关系是有效的。
- 香蕉 /
- 亲缘关系 /
- ISSR /
- 聚类分析
Abstract: Genetic diversity and relationship among 35 banana germplasms involving 3 genotypes (i.e., AAA, AA and ABB) were studied by using the Inter-Simple Sequence Repeat (ISSR) method.As a result, 6 ISSR primers were selected, 38 bands amplified between 200 bp and 2,000 bp, 27 bands detected for polymorphic DNA, and the percentage of polymorphic DNA 71.05% of polymorphic DNA were found.The genetic distances among the banana cultivars were between 4.30% and 33.33%.A cluster analysis was performed applying the unweighted pair group method on arithmetic averages.The banana germplasms were grouped into 6 clusters based on the genetic distance of 25%.Except for Gongjiao (AA) and Jinfen1 (ABB), which were grouped into Cluster III and IV, respectively, the remaining 33 germplasms were classified into 4 clusters, in which Cluster I contained 22 germplasms from different localities and genetypes (AAA and ABB).All germplasms with genotypes AAA and ABB could not be divided into 2 clusters.There appeared no close genetic relationship among some banana germplasms with same genotypes.The germplasms could be discriminated by the 6 selected primers, and ISSR was considered as an effective method for studying genetic relationship among different banana germplasms resources.
- Banana (Musa spp) /
- genetic relationship /
- ISSR /
- cluster analysis