PCR and amplification of ITS and 5.8S ribosomal DNA for Fusarium genus detection and identification
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摘要: 通过对ITS/5.8SrDNA区域的PCR和巢式PCR扩增检测真菌DNA,同时评估了PCR和巢式PCR技术的敏感性和特异性。证实了通过PCR和巢式PCR扩增不同种真菌菌株ITS/5.8SrDNA的可行性,通过PCR产物的测序及GenBank比对,能成功检测出该真菌菌株。说明通过对真菌ITS/5.8SrDNA区域的扩增是一种快速检测真菌的方法。Abstract: Internal transcribed spacer 1 (ITS1),ITS2 and 5.8S rDNA were amplified by PCR and semi-nested PCR to detect Fusarium DNA.Meanwhile, PCR and semi-nested PCR of the region were employed to evaluate the sensitivity and specificity of the method.The amplified DNA was sequenced and blasted against sequences in the GenBank at the National Center for Biotechnology Information.The results proved that this DNA amplifying technique was able to distinguish different species of Fusarium genus.Therefore,amplifying and sequencing of ITS/5.8S rDNA could be a rapid,applicable method for identifying Furisarum genus.
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Keywords:
- rDNA sequence /
- fungal pathogens /
- semi-nested PCR
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