Abstract: The polymorphism of FSHβ gene was detected by PCR amplication,and the correlation between the genotypes of FSHβ gene and reproductive traits was analyzed.The result showed that the frequency of A allele of FSHβ gene in Large white,Landrace and their crossbred were lower as 0.2133,0.1615 and 0.1405 respectively,while the frequency of B allele of FSHβ gene were higher as 0.7867,0.8385 and 0.8595 respectively.There were significant differences between the genotypes of the polymorphism site in Number Born Alive (NBA) and Birth Weight (BW) in first parity sows (P0.05),and were not significant difference in Total Number Born (TNB) and Number Born Weaning (NBW) (P0.05);and there were not significant differences between the genotypes of the polymorphism site with TNB,NBA,BW and NBW in Later parity.