Abstract: The PCR amplified ITS fragments were analyzed by restriction endonuclease digestion (RFLP).Results showed that the RFLP patterns of S.halepensem (L.) Pers which yielded two bands around 650 bp and two bands around 200 bp.But significantly different from the RFLP patterns of S.sp.and S.propinguum (Kunth.) Hitche which yielded one band around 650 bp and one band around 200 bp.The results were consistent with the fact which S.halepensem had two selective identify sites of PmaC I while S.sp.and S.propinguum each had one.Therefore, it could differentiate S.propinguum, S.sp.and S.halepensem (L.) Pers by PmaC I.