Abstract: By using DNA recombination technology,nucleocapsid gene from swine transmissible gastroenteritis virus was lined with the gene fragment of the maximum local hydrophilic antigenic determinant of nucleocapsid gene from porcine epidemic diarrhea virus.The recombinant gene was cloned into the prokaryotic expression vector pET-32a(+).Then,the prokaryotic expression plasmid with the bivalent gene to prevent piglet’s coronaviral diarrhea was constructed.After transforming the plasmid into E.coil BL21(DE3),the bacteria were induced by IPTG with SDS-PAGE analysis showing the bivalent gene expressed efficiently,as well as,the western-blot test expressing the fusion protein.