Abstract:
Objective Flavonoids and expressions of genes related to flavonoid synthesis in the leaves and vines of Ipomoea batatas L. vegetable plants were analyzed.
Method At various growth periods, in parts of a leaf and sectioned vine of the sweet potato plant commonly used as fresh vegetable for consumption, contents of flavonoids were determined using spectrophotometric colorimetry, composition of specific flavonoids detailed by ultra performance liquid chromatography-tandem time-of-flight mass spectrometry (UPLC-TOF-MS), and expressions of flavonoid biosynthesis genes detected by qRT-PCR.
Result (1) The total flavonoids in different parts of the sweet potato leaf as well as vine section varied quantitatively in the rank of mesophyll ＞ vein ＞ vine section ＞ petiole. (2) The contents rose as the plant grew. (3) Five major flavonoids in these parts were rutin, isodendroside, dendrobin, naringenin, and kaempferol, with isoquercitrin being the most and rutin the next. And (4) IbFLS appeared to be crucial in the flavonoid formation in a sweet potato plant.
Conclusion Both content and composition of the flavonoids differed in parts of a leaf and vine section and changed with the plant growth. Isoquercitrin was the predominant flavonoid, and IbFLS the key gene that regulated synthesis of the bioactive ingredient in I. batatas L.
下载: