Abstract: Layers from one of the chicken houses in Fujian showed egg laying reduction and some died.Hemangioma was observed on their claws.Using a real-time florescence quantitative polymerase chain reaction method,ALV-J was found in the tissue and blood samples of the infected layers.The sequence analysis further indicated that the viruses were highly homologous to those reported previously at home and abroad.This was the first time the existence of ALV-J in domestic layers in Fujian was confirmed by means of a pathogenic microbiological diagnostic method.