反相高效液相色谱法同时测定枇杷中的某些有机酸

Determination of some organic acids in the fruits of loquat by RP-HPLC

摘要: 采用反相高效液相色谱法(RP-HPLC)测定枇杷果实中的有机酸成分和含量,结果表明,在以Waters μBondapakTMC18为色谱柱,使用紫外检测器(波长214 nm),0.04 mol·L-1 KH2PO4-H3PO4缓冲液作流动相(pH 2.4),流速为0.8 ml·min-1,柱温为30℃的色谱条件下,枇杷有机酸能得到良好分离,其中9种有机酸标准曲线相关系数达0.999 3以上,平均回收率为85%-102%,变异系数RSD5.05%。

Abstract: A RP-HPLC method with UV detector was established to determine the organic acids in the fruits of loquat.The chromatographic conditions were Waters μBondapakTMC18 (3- 9mm i.d×300mm, 10μm) used for column (at 30℃)with 0.04 mol·L-1 KH2PO4 -H3PO4 buffer (pH2.4) as mobile phase, and flow rate 0.8 ml·min-1, detector UV-214 nm.The correlation coefficients of the calibration graphs were 0.9993-0.9999.The averaged recoveries were 85% -102%, RSD5.05%.