Study on in vitro culture and rapid propagation of Pseudostellaria heterophylla growing point
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摘要: 利用太子参0.2-0.5 mm大小的生长点进行离体培养获得了再生植株,通过正交试验和单因子试验,确定了茎尖诱导成芽初代培养基为MS+6-BA 2 mg·L-1+NAA 0.2 mg·L-1+GA3 0.1-0.2 mg·L-1+蔗糖30 g·L-1;适于愈伤组织及不定芽增殖的继代培养基为MS+6-BA 1.5 mg·L-1+NAA 0.01 mg·L-1+蔗糖30 g·L-1;再生植株的快速繁殖可通过愈伤组织不断分化不定芽和单茎节切段繁殖2种途径进行;单茎节切段快繁培养基为MS基本培养基;适于试管微形参形成的培养基为MS+蔗糖60 g·L-1,pH 6.0,最适培养条件为20℃, 光照16 h·d-1;生根培养基为MS+蔗糖30 g·L-1。在气温15-20℃、空气相对湿度90%条件下,试管苗定植于细沙土中的成活率达98.6%;太子参茎尖组培苗田间花叶病发病率比普通苗低94.75%。Abstract: The regeneration plants were acquired by culturing in vitro the growing points (in 0.2-0.5 mm length) of Pseudostellaria heterophylla.The orthogonal experiments and single factor experiments showed that the optimum medium for calli induction and shoot buds from calli was MS+6-BA 2 mg·L-1+NAA 0.2 mg·L-1 + GA30.1-0.2 mg·L-1+sugar 30 g·L-1.The medium suitable for calli subculture was MS+6-BA 1.5 mg·L-1 + NAA 0.01 mg·L-1+sugar 30 g·L-1.The plantlets’propagation had been done by 2 ways i.e.continuous calli differentiation and plantlets cuttage propagation.Basic MS medium was suitable for plantlets cuttage propagation.Medium MS (pH 6.0) containing 60 g·L-1 sugar was suitable to induce micro-root tuber, under the condition of 20℃ and 16 hours of light everyday.Medium MS containing 30 g·L-1 sugar was suitable for roots differentiation.The survival rate of the tissue culture plantlets exposed to air for 10-15 days reached 98.6% in the sandy soil under the condition of 15-20℃ and the relative moisture more than 90%.The mosaic disease rate of virus-free roots was 94.75 % lower than that of conventional culture system in the field.
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