青枯病生防菌ANTI-8098A的微胶囊化技术

Microcapsule manufacture for bio-controlling strain ANTI-8098A(Bacillus cereus)for bacterial wilt disease

摘要: 以明胶和阿拉伯胶为壁材,通过复凝聚法进行青枯病生防菌ANTI8098A微胶囊的制备,研究壁材浓度、生防菌ANTI8098A发酵液添加浓度、搅拌速度、酸碱度和甲醛固化条件对微胶囊粒径、形状的影响.试验结果表明,用2.5%明胶和2.5%阿拉伯胶为壁材制备青枯病生防菌ANTI8098A微胶囊,条件为pH值4.0、生防菌发酵液浓度10%～30%、搅拌速度400 r·min-1、固化时添加1%的甲醛溶液,生防菌的包被率可稳定在70%左右,微胶囊为圆形,平均直径为30.8~57.3 μm,生防菌微胶囊萌发率达93%以上.

Abstract: Microcapsule for bio-controlling strain ANTI-8098A(Bacillus cereus)to control bacterial wilt disease was manufactured by coagulating method, using glutin and arabic gum as shell-materials. The effects of shell-material concentration. ANTI-8098A concentration,stirring speed and formaldehyde solidification on formation of mi-crocapsule were studied. Results showed that using 2.5% glutin. 2.5 arabic gum with 10%-30% ANTI-8098A under conditions of stirring speed at 400 r·min-1,1% formaldehyde for solidification could result in round micro-capsule with diameter of 30.8-57.3μm and 70% retention ratio of the bacteria, the germination rate of microcap-sule of bio-controlling strain ANTI -8098A was over 93%.