Abstract: The protein content and amino acid composition in fruitbodies of Pleurotus eryngii were analysed,the protein nutrient value of the fruitbodies of Pleurotus eryngii strains Pe5 and Pe6 was assessed by the international common method. The result showed that the strain Pe5 was better than the strain Pe6 with higher nutrient value. The cotton seed hull compost was not suitable for the strain Pe5 cultivation. While Miscanthus floridulus could substitute to sawdust and bagasse as compost material for Pleurotus eryngii.