嗜水气单胞菌<i>β-hem</i>A重组菌表达产物ISCOMs的研制

嗜水气单胞菌β-hemA重组菌表达产物ISCOMs的研制

基金项目:

国家863资助项目(2003AA622050);福建省科技厅资助项目(2000Z077)

详细信息

Institute of Animal Husbandry and Veterinry Medicine, Fujian Academy of Agricultral Sciences Fuzhou Fujian 350003, China

摘要: 嗜水气单胞菌β-hemA 重组菌 PCB 的培养上清液经硫酸铵梯度沉淀粗提毒素蛋白质,并在pH2.5的柠檬酸缓冲液里酸化处理以暴露其疏水区,经Mega-1O裂解后与 Quil A 结合制备 ISCOMs,电镜下可观察到30～40nm 的多面体的笼格状颗粒结构。经鳗鱼的免疫保护试验证实,ISCOMs组(每尾40~80pg)保护率高达100%,同剂量的溶血素+弗氏佐剂组可达62.5%,未加佐剂的溶血素只有50%。
- 嗜水气单胞菌 /
- 免疫刺激复合物(ISCOMs) /
- 免疫
Abstract: A method for preparing the immuno-stimulating complexes(ISCOMs)with theβ-hemolysin(β-HemA) and Quil A was described,in which the β-hemolysin of Aeromonas hydrophila was extracted by ammonium sulphate gradient precipitation and pretreated with an acidic pH(2.5)to reveal hydrophobic regions,and then was mixed with the Quil A and other reagents.The ISCOMs was a cagelike Structure with a diameter of 30—40 nm by negative contrast electron microscopy.Several groups of eel were immunized with β-HemA-ISCOMs,β-HemA-CFA and same amount of β-hemolysin without adjuvant,respectively,and followed by challenge with a virlent bacterial strain TPS-30.The protecion rates of that were 100%,62.5%,and 50%,respectively.The result indicated that the eel immunized with β-HemA-ISCOMs were effectively protected against the challenge.
- Aeromonas hydrophila /
- Immune stimulating complexes (ISCOMs) /
- Immunology

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