Abstract: The studies of symmetrical fusion of protoplasts isolated from Cuixiu mini cucumber (Cucummis sativus L. ) and fig-baf gourd (Cucurbita ficifolia Bouche) were carried out. The results indicated that the conditions could lead to the best effect of cell fusion were using 0.5 mol·L-1 mannitol as fusional solution and setting the alternating current field intensity at 25 V·cm-1, the alternating current frequency at 2000 kHz, the direct current intensity at 200 V·cm-1, the direct current pulse width at 40μs and 3 direct pulse times. After cultured in shallow-layer liquid medium, large numbers of callus were regenerated. The genetic identification of callus and the plant regeneration were also evaluated.