• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

三峡库区抗稻瘟病水稻资源基因的分子检测与分析

Blast Resistance Genes in Rice Cultivars from Three Gorges Reservoir Area

    摘要
  • 摘要:
    目的 通过分析70份三峡库区水稻材料中12个抗稻瘟病基因的分布与表达特征，筛选具有区域适应性的有效抗稻瘟病基因。
    方法 以70份水稻品种为试验材料，在万州和恩施病圃进行田间稻瘟病抗性鉴定，并采用PARMS SNP分型技术、实时荧光定量PCR对12个抗稻瘟病基因进行检测分析。
    结果 70份材料在万州和恩施病圃的稻瘟病抗性基本处于中抗及以上水平；但水稻材料在恩施病圃存在感病的情况，且在恩施病圃的抗性明显低于万州病圃。分子生物学检测结果表明，12个抗稻瘟病基因在70份供试水稻材料中均有分布，供试材料携带抗性基因数在5～12个，其中携带9个抗性基因的最多，达28.99%；携带5个和12个抗性基因的材料最少，分别占2.9%和1.45%。荧光定量PCR分析结果表明，Pb1、Pita、Pit和Pi5基因在供试材料中基因表达量最高，且在供试材料中主要表现为基因上调，推测有可能为三峡库区抗稻瘟病的有效抗病基因。
    结论 通过田间抗性调查与12个抗稻瘟病基因的分子标记检测结果，推测Pb1、Pita、Pi5和Pit基因为三峡库区水稻抗稻瘟病的有效抗病基因，为三峡库区抗稻瘟病品种的选育提供理论支撑和新思路。

     

    Abstract:
    Objective Blast resistance genes in the rice cultivars from Three Gorges Reservoir area with high regional adaptability were identified and analyzed.
    Methods Seventy rice cultivars from the blast-infected nurseries at Wanzhou and Enshi cities near the Three Gorges Reservoir were collected to test for resistance on the disease. Genes identified to be resistant to blast were analyzed using the PARMS SNP typing technique and RT-qPCR.
    Results Other than a few from Enshi that were susceptible to blast, all 70 of the collected rice cultivars displayed medium to superb resistance to the disease. Overall, those came from Wanzhou were significantly more resistant than their Enshi counterparts. Twelve blast resistance genes were found, and 28.99% of the 70 cultivars carried 9 of the genes, while 2.9% carried 5 and 1.45% carried all. Pb1, Pita, Pit, and Pi5 were the most highly expressed among the resistance genes. They could be the key players in the disease resistance of rice in the area.
    Conclusion Of the 12 genes with medium to superb resistance to blast on rice plants, Pb1, Pita, Pi5, and Pit could be of interest for breeding purpose.

     

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