• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

CMV和LSV侵染本氏烟及兰州百合发生协生作用的影响因素

Factors Affecting Synergistic CMV/LSV Co-infection on Nicotiana benthamiana and Lilium davidii Duch

    摘要
  • 摘要:
    目的 探明黄瓜花叶病毒（cucumber mosaic virus, CMV）和百合无症病毒（lily symptomless virus, LSV）复合侵染兰州百合发生协生作用的影响因素。
    方法 以本氏烟（Nicotiana benthamiana）和兰州百合（Lilium davidii Duch）为寄主，以兰州百合病毒病样中提纯的CMV和LSV为供试病毒，采用摩擦接种法和DAS-ELISA法，对CMV和LSV复合接种侵染本氏烟发生协生作用的最适浓度、温度、相对湿度及接种的先后次序进行研究，筛选出发生协生作用的最佳条件，在兰州百合上接种、培养，利用RT-qPCR对接种百合新叶中两种病毒外壳蛋白（coat protein, CP）基因的相对表达量进行检测和协生作用验证。
    结果 适于CMV+LSV复合侵染发生协生作用的最佳条件为接种浓度3.57%和12.50%CMV（14.29%）和LSV（16.67%）、温度25℃、RH 60%、接种顺序为先接种CMV 48 h后再接种LSV，复合侵染较单独侵染发病更早，主要表现为未接种生叶片出现重度花叶、失绿、明脉等症状，生长后期上部叶片出现卷曲，CMV和LSV病毒浓度分别为10.37 ng·mL−1和3.66 ng·mL−1，是两种病毒单独侵染时的 1.45倍和1.11倍，且CMV的浓度明显高于LSV，为被协生病毒，LSV为协生病毒；接种百合鳞片后，在筛选出的最适条件下培养60 d，经RT-qPCR检测发现，两种病毒复合侵染的百合新叶中CMV-CP的相对表达量显著高于LSV-CP和两种病毒单独侵染中CMV-CP、LSV-CP的相对表达量，表明两种病毒复合侵染百合发生协生作用的条件与侵染本氏烟的相一致。
    结论 先接种浓度3.57%的CMV 48 h后再接种浓度12.50%的LSV的本氏烟、兰州百合，在温度25℃、RH 60%条件下最有利于协生作用的发生，两种病毒含量均高于单独接种，且CMV含量显著提高，为被协生病毒，LSV为协生病毒。研究结果对进一步明确CMV+LSV复合侵染本氏烟、百合等寄主的协生作用发生规律以及指导病毒病有效防治具有重要意义。

     

    Abstract:
    Objective Factors affecting the synergism of co-infection of cucumber mosaic virus (CMV) and lily symptomless virus (LSV) on Lilium davidii Duch in comparison to Nicotiana benthamiana were studied.
    Methods  CMV and LSV virions purified from diseased lily plants were used to infect N. benthamiana and L. davidii by the friction inoculation and DAS-ELISA methods. Same concentration, temperature, relative humidity, and sequence of CMV and LSV inoculations optimal for a synergistic effect on N. benthamiana were applied on L. davidii. Relative expressions of the two viral coat protein (CP) genes in the inoculated new leaves of the plants were determined with synergistic effect examined by RT-qPCR.
    Results The optimum synergistic effect was observed by using 3.57% CMV and 12.50% LSV CMV ( 14.29 % ) and LSV ( 16.67 % ) to inoculate CMV 48 h prior to LSV at 25°C and 60% RH. Disease symptoms appeared sooner on the co-infected plants than those individually inoculated with CMV or LSV. The major symptoms included 重度花叶、失绿 (loss of green color?)、明脉 and curled leaves on top of the plants during the late growth period. The contents of CMV and LSV in the infected plants were 10.37 ng·mL-1 and 3.66 ng·mL-1, respectively, which were 1.45 and 1.11-folds those in the plants of single infection. The CMV content was significantly higher than LSV, as CMV being the passive synergistic virus, while LSV is the synergistic virus. After inoculating in lily scales and being cultured under the optimal conditions for 60 d, the relative expression detected by RT-qPCR on CMV-CP in the new leaves of L. davidii co-infected with the two viruses was significantly higher than those on LSV-CP or CMV-CP and LSV-CP in the leaves of lily plants infected by the two viruses individually. The co-infection synergisms on L. davidii and on N. benthamiana appeared to be identical.
    Conclusion N. benthamiana and L. davidii inoculated with 3.57% CMV for 48 h followed by 12.50% LSV at 25°C and 60% RH were shown to have the same synergism. The contents of the two viruses were higher in co-infected plants than those in single inoculated counterparts, and the passive synergistic CMV significantly increased in concentration in comparison to the synergistic LSV.

     

    • HTML全文
    • 参考文献(35)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回