Abstract: Antifungal activities of 8 strains of Trichoderma spp. on Botrytis cinerea in Fujian was studied. By means of confrontation culture, all of the strains were shown to effectively inhibit the mycelial growth of the pathogen with inhibition rates ranging from 86.83% to 91.39%. The fermentation broths of the Trichoderma strains differed significantly in their inhibiting effects on hyphal growth of the pathogen. The broth from FJ2006-8 was found to render the highest inhibition rate of 39.44% among all. All tested strains produced volatile as well as non-volatile metabolites. The non-volatile metabolites produced by FJ2006-8 showed the greatest inhibitory effect, reaching a rate of 61.09%, while the volatiles from T05-58 exhibited the highest rate of 22.95%. The results would provide the needed information to develop a biocontrol agent from Trichoderma for the disease on tomato plants caused by the gray mold.