养猪发酵床垫料微生物类群结构特性分析

蓝江林; 栗丰; 刘波; 史怀; 黄素芳

doi:10.19303/j.issn.1008-0384.2016.06.016

养猪发酵床垫料微生物类群结构特性分析

蓝江林¹,
栗丰^1,2,
刘波^1, ,,
史怀¹,
黄素芳¹

1.
福建省农业科学院农业生物资源研究所, 福建福州 350003;
2.
福建农林大学, 福建福州 350108

基金项目:

国家科技支撑计划（2012BAD14B15）;福建省科技重大专项（2012NZ0002）;公益性行业（农业）科研专项（201303094）

详细信息

作者简介:
蓝江林(1972-),女,博士,研究员,主要从事应用微生物研究(E-mail:lanfz2008@163.com)

通讯作者:
刘波(1957-),男,博士,研究员,主要从事农业微生物研究(E-mail:liubofaas@163.com)

中图分类号: S828
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出版历程
- 收稿日期: 2016-01-28
- 修回日期: 2016-04-10
- 刊出日期: 2016-06-27

Analysis on Spatial Distribution of Microbes Population in the Fermentation Bed

1.
Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian 350108, China

摘要

摘要: 采用定点取样、平板分离的方法，分析养猪发酵床垫料微生物的结构特性。结果表明，在30个样点5次取样过程中，微生物含量总体为细菌放线菌真菌。细菌含量变化幅度最大[（33.72～197.23）105cfug-1]，真菌含量变化相对较小[（7.87～48.64）103cfug-1]，而放线菌含量变化稳定[（20.14～33.18）104cfug-1]。细菌含量类群Ⅰ（大于70.00105cfug-1）平均9.2个样点、占总样点的28.67%；类群Ⅲ（小于30.00105cfug-1）平均为8.6个样点，占总样点的30.67%；类群Ⅱ[（30.00～70.00）105cfug-1]平均为12.2个样点，所占比例最大，为40.67%。真菌类群Ⅰ（大于70.00103cfug-1）平均仅3.8个样点，所占比例最低，为12.67%；类群Ⅱ[（30.00～70.00）103cfug-1]平均为6.4个样点，所占比例为21.33%；类群Ⅲ（小于30.00103cfug-1）平均19.8个样点，所占比例高达66.00%。放线菌3种类群所占比例与真菌类似，类群Ⅰ（大于70.00104cfug-1）平均仅1.4个样点，所占比例最低，仅为4.67%；类群Ⅱ[（30.00～70.00）104cfug-1]平均8.4个样点，所占比例为28.00%；类群Ⅲ（小于30.00104cfug-1）平均20.2个样点，所占比例高达67.33%。
- 发酵床垫料 /
- 微生物类群 /
- 空间结构
Abstract: Spatial distribution of microbes in the fermentation bed was analyzed by sampling and culturing, the results indicated that the amount of bacterium was highest, the second was actinomycete, the amount of fungi was lowest. In all of the samples,amplitude of variation of bacterial content was the biggest with(33.72~197.23)105cfug-1, fungal content changes are relatively small with(7.87~48.64)103cfug-1, and Actinomyces content was stability with(20.14~33.18)104cfug-1. GroupⅠ(more than 70.00105cfug-1)of bacteria content was averaged 9.2 sample points, accounting for 28.67% of the total sample; group Ⅲ(less than 30.00105cfug-1)was averaged 8.6 points, accounting for 30.67% of the total sample. Group Ⅱ (30.00~70.00)105cfug-1 was averaged 12.2 points, accounted for the largest proportion of 40.67%. Fungal group I(more than 70.00103cfug-1)was averaged only 3.8 points, the lowest proportion for 12.67%, Group Ⅱ(30.00~70.00)103cfug-1 was averaged 6.4 points, the proportion was 21.33%, group Ⅲ (less than 30.00103 cfug-1) was averaged 19.8 points, a whopping 66.00%.The proportion of actinomyces was similar to type of fungi, group I (more than 70.00104cfug-1)was averaged just1.4 sample points, the lowest proportion, only 4.67%.Group Ⅱ(30.00~70.00)104cfug-1 was averaged 8.4 points and percentage 28%, Group Ⅲ(less than 30.00104cfug-1)was averaged 20.2 sample points, a whopping 67.33%.
- litter in fermentation bed /
- microbes populationg /
- spatial distribution

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