Comparing Three Methods in Determining Hydrolytic Degree of Cottonseed Protein
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摘要: 介绍了一种比较简单可行、适用于棉籽蛋白水解度的测定方法OPA法。选用中性蛋白酶将棉籽蛋白水解成棉籽多肽,通过新建立的方法测定不同酶解时间棉籽蛋白水解液的水解度,并与文献所报道的甲醛滴定法和茚三酮比色法进行比较。结果表明,与甲醛滴定法和茚三酮比色法相比,OPA法更简便、更快速,且灵敏度高、可重复性强、对环境友好,是3种方法中最适合测定棉籽蛋白水解液水解度的方法,同时该方法也适用于饲料及食品行业中其他蛋白水解度的测定。Abstract: This paper introduced a method that based on the reaction of primary amino groups with o-phthaldialdehyde (OPA) for determining the hydrolytic degree of cottonseed protein.Neutral protease was used to hydrolyze cottonseed protein and the degree of hydrolysis (DH) was compared with three assay methods, i.e.o-phthaldialdehyde method, formaldehyde titration method and ninhydrin colorimetric method.Comparing with formaldehyde titration and ninhydrin colorimetric methods, results showed that OPA method performed more accurate and faster in analyzing, easier to handle, repeatable in results and friendly for environment.It is recommended that OPA method is an appropriate method for evaluating in hydrolytic degree of cottonseed protein.
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Keywords:
- hydrolytic degree /
- cottonseed protein /
- o-phthaldialdehyde method
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NIELSEN P M, PETERSEN D, DAMBMANN C. Improved method for deter ming food protein degree of hydrolysis[J]. Journal of Food Science, 2001, 5: 642~643.
KRAMAC M, AMAROWICZ R,KOSTYRA H. Trypsynowa hydroliza bialek grochu[J]. In Materialy XXVⅡ Sesja Naukowa Komitetu Technologiii Chemii Zywnosci PAN, 1996:113-l18.
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