樱桃番茄种质资源遗传多样性的RAPD分析

基金项目:

福建省科技厅重点项目(2005N026)

详细信息

Genetic diversity of Lycopersicon esculentum var.cerasiforme germplasms by RAPD analysis

1.
Institution of Cultivation and Rotation, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 从200个随机引物中筛选出27个引物,对32个樱桃番茄品种进行RAPD分析,并对樱桃番茄遗传多样性和分类进行探讨和研究,结果显示,27个引物共扩增出207条DNA谱带,其中多态条带139条,多态性程度为 67.15%;平均每个引物产生7.67条,每个引物可扩增出2-12条多态性谱带,谱带大小为270-2000 bp;遗传距离D值在0.33水平上,能将供试材料聚为3类:野生种聚成一类,果皮为黄色和少数红色品种聚成一类,全部是红色聚成一类;此外,还发现从重要特征观察,樱桃番茄品种表现出一定的聚集趋势。
- 樱桃番茄 /
- RAPD /
- 遗传多样性 /
- 聚类分析
Abstract: The genetic polymorphisms in Lycopersicon esculentum var.cerasiforme of thirty-two cultivars were investigated by using random amplified polymorphic DNA markers (RAPD).Twenty-seven primers selected from two handred primers amplified 207 DNA fragments and 139 bands showed polymorphisms that occupied 67.15% of the whole amplified bands.Each primer could amplify 2 to 12 polymorphic bands, with an average of 7.67 bands.The length of most amplified fragments ranged from 270 to 2000 bp.Cluster analysis showed that the 32 cultivars could be classified into 3 groups at the level of D 0.33, which evidently showed the variations in some important traits of Lycopersicon esculentum var.cerasiforme.
- Lycopersicon esculentum var.cerasiforme /
- RAPD /
- genetic diversity /
- germplasms

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