In Vitro Micropropagation of Pendent Cymbidiumwith Clustered Shoots

Lateral buds were used as the explants for the study on tissue culture of pendent Cymbidium.It showed that by using 72%ethanol,0.1% mercuric chloride and 15%sodium hypochlorite to disinfect the lateral buds three times,the bud survival rate could be as high as 56%.The disinfection was especially effective in springtime.The induction rate of the clustered shoots was up to 100% with numerous differentiated buds when 6-BA was applied at the rate of 4.0mg·L-1.During the proliferating period of the clustered shoots,the 6-BA treatment was a key factor that produced the best effect with a proliferating coefficient of clustered shoots of 3.4and strong buds.For the development in the rooting stage,on the other hand,a 1/2MS medium containing NAA 2.0mg·L-1+banana slurry 50g·L-1+AC 1.0g·L-1appeared to be most desirable.And,moss seemed to be the best base material for the cultivation of the pendent Cymbidiumplantlets.