Optimizing Medium for Flavonoid-producing <i>Carpesium Macrocephalum</i> Culture

LI Yu-ping; GONG Ning

doi:10.19303/j.issn.1008-0384.2019.06.005

Volume 34 Issue 6

Sep. 2019

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LI Yu-ping, GONG Ning. Optimizing Medium for Flavonoid-producing Carpesium Macrocephalum Culture[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 652-659. doi: 10.19303/j.issn.1008-0384.2019.06.005

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LI Yu-ping, GONG Ning. Optimizing Medium for Flavonoid-producing Carpesium Macrocephalum Culture[J]. Fujian Journal of Agricultural Sciences, 2019, 34(6): 652-659. doi: 10.19303/j.issn.1008-0384.2019.06.005

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Optimizing Medium for Flavonoid-producing Carpesium Macrocephalum Culture

doi: 10.19303/j.issn.1008-0384.2019.06.005

LI Yu-ping^{1, 2, 3
,},
GONG Ning^{4
,
,}

1.
College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China
2.
Graduate School, Qinghai University, Xining, Qinghai 810016, China
3.
Technology and Engineering Center of Biopesticide of Shaanxi Province, Northwest A & F University, Yangling, Shaanxi 712100, China
4.
College of Chemistry & Pharmacy, Northwest A & F University, Yangling, Shaanxi 712100, China

Received Date: 2019-04-18
Rev Recd Date: 2019-05-24
Publish Date: 2019-06-28

Abstract

Abstract

Objective Formulation of the medium for Carpesium macrocephalum culture to produce flavonoids was optimized from the one obtained by a previous study. Method Using NT+NAA 1.0 mg·L^-1+0.2 mg·L^-1 6-BA as the base, the medium optimizations were firstly carried out on the macro-elements, NH₄⁺, NO₃^- and K⁺, then the micro-elements, MoO₄^2-, Zn²⁺, BO₃^3-, Co²⁺, Cu²⁺, I^-, and Mn²⁺ and followed by the added 2, 4-D, KT and GA₃. The cellular growth as well as the flavonoids yield in the culture suspension were monitored for evaluation. Result With the optimized addition of the macro- and micro-elements plus KT or GA₃, the flavonoids produced by C. macrocephalum culture significantly increased (P < 0.05), but reduced by the presence of 2, 4-D. The finalized medium consisted of the base with added 15.46 NH₄⁺ mmol·L^-1, NO₃^- 14.10 mmol·L^-1, K⁺ 19.10 mmol·L^-1, MoO₄^2- 3.0 μmol·L^-1, Zn²⁺ 0.06 mmol·L^-1, BO₃^3- 0.4 mmol·L^-1, Co²⁺ 0.2 μmol·L^-1, Cu²⁺ 0.4 μmol·L^-1, I^- 10 μmol·L^-1, Mn²⁺ 0.2 mmol·L^-1, and KT 0.2 mg·L^-1. The resulting flavonoids content in the culture suspension was 2.05%, which was 1.68-fold of control, and the flavonoids yield 517.87 mg·L^-1, which was 1.80-fold of control. Conclusion By further modifying the previously obtained medium, the flavonoids produced from the C. macrocephalum culture significantly increased.
- macroelements,
- microelements,
- phytohormone,
- flavonoids,
- Carpesium macrocephalum

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References(30)

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