Structure and Genome Sequence of <i>Papilio polytes</i> Mitochondria

WENG Qingfen; ZHAO Zhuo; LIU Chenyang; ZHAI Qing

doi:10.19303/j.issn.1008-0384.2022.003.011

Volume 37 Issue 3

Mar. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(3): 364-370

WENG Q F, ZHAO Z, LIU C Y, et al. Structure and Genome Sequence of Papilio polytes Mitochondria [J]. Fujian Journal of Agricultural Sciences，2022，37（3）：364−370 doi: 10.19303/j.issn.1008-0384.2022.003.011

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WENG Q F, ZHAO Z, LIU C Y, et al. Structure and Genome Sequence of Papilio polytes Mitochondria [J]. Fujian Journal of Agricultural Sciences，2022，37（3）：364−370 doi: 10.19303/j.issn.1008-0384.2022.003.011

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Structure and Genome Sequence of Papilio polytes Mitochondria

doi: 10.19303/j.issn.1008-0384.2022.003.011

Plant Protection College, Henan Agricultural University, Zhengzhou, Henan　450002, China

Received Date: 2021-10-25
Rev Recd Date: 2022-03-10

Available Online: 2022-04-24

Publish Date: 2022-03-28

Abstract

Abstract

Objective Adults and larvae of Papilio Polytes were studied with the complete mitochondrial genome sequenced for the butterfly genomics library collection. Method According to the morphological characteristics of wing markings, sequences of the mitochondrial genomes of P. Polytes were determined using the second-generation Illumina Hiseq 4 000 high-throughput sequencing technology. Result The wing markings of P. polytes polytes was found significantly different from those of P. polytes tibetanus’s. The length of complete mitochondrial genome was 15 267 bp (GenBank accession no. MZ188895) consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a control region. The average contents of A+T was 80.6% of the entire sequence representing a significant A+T deflection that had 11 gene intervals and 12 gene overlaps. Except Cox1, all protein coding genes started with ATN, whereas Cox2 ended with codon T. The nucleotide composition showed a significant AT skew. The relative synonymous codon usage (RSCU) of UUA was the highest one. Other than trnS1, the transfer RNAs had the typical clover-leaf-like structure with U-U or U-G base mismatched in the secondary structure and 9 transfer RNAs without mismatch. The A+T average content was the highest in control region at 94.5%. Conclusion Morphologically, P. polytes polytes and P. polytes tibetanus mainly differed in location and distribution of the elements. The structures and sequences of the genomes of P. Polytes mitochondria as determined were in line with those of Lepidoptera insects.
- Papilio polytes,
- characteristics,
- mitochondrial genome,
- sequencing

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References(16)

References

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