Interacting Proteins with JsMYB305 Transcription Factor in <i>Jasminum sambac</i>

HU Li; WAN Chao; ZHANG Qu; CHEN Qingxi; WU Binghua; YUAN Yuan

doi:10.19303/j.issn.1008-0384.2022.009.004

Volume 37 Issue 9

Sep. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(9): 1135-1144

HU L, WAN C, ZHANG Q, et al. Interacting Proteins with JsMYB305 Transcription Factor in Jasminum sambac [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1135−1144 doi: 10.19303/j.issn.1008-0384.2022.009.004

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HU L, WAN C, ZHANG Q, et al. Interacting Proteins with JsMYB305 Transcription Factor in Jasminum sambac [J]. Fujian Journal of Agricultural Sciences，2022，37（9）：1135−1144 doi: 10.19303/j.issn.1008-0384.2022.009.004

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Interacting Proteins with JsMYB305 Transcription Factor in Jasminum sambac

doi: 10.19303/j.issn.1008-0384.2022.009.004

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-06-13
Rev Recd Date: 2022-09-01

Available Online: 2022-10-05

Publish Date: 2022-09-30

Abstract

Abstract

Objective Aroma-regulating mechanism of the transcription factor JsMYB305 associated with terpene synthetases gene of jasmine flower was investigated. Method From the jasmine floral petal yeast database a two-hybrid library was constructed using the white buds and fully open flowers. Proteins that interacted with JsMYB305 were identified and verified by the yeast two-hybrid method. Result The full length of JsMYB305 was significantly self-activated, and the fragments of different lengths were amplified for verification. At a coding length less than or equal to 510 bp, JsMYB305 showed no self-activation. Applying a 510 bp pGBKT7-JsMYB305 as a bait, an interacting protein was obtained from the yeast two-hybrid library and identified to be salicylic acid carboxyl methyltransferase (SAMT). The yeast-directed two-hybrid validation indicated that JsMYB305 interacted with JsSAMT. Conclusion Since SAMT presents in the biosynthetic pathway of phenylpropane/propane, the JsMYB305 transcription factor was believed to also relate to the synthesis of similar aromatic substances in jasmine flowers.
- Jasminum sambac,
- MYB transcription factor,
- yeast two-hybrid,
- interaction protein

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References(48)

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