Molecular Mechanism of <i>traf6 like</i> Gene in Innate Immunity of <i>Procambarus clarkii</i>

LIN Sihan; YANG Bingbing; LI Qianqian; SHEN Xiuli; DU Zhiqiang

doi:10.19303/j.issn.1008-0384.2022.010.001

Volume 37 Issue 10

Oct. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(10): 1245-1249

LIN S H, YANG B B, LI Q Q, et al. Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1245−1249 doi: 10.19303/j.issn.1008-0384.2022.010.001

Citation:

LIN S H, YANG B B, LI Q Q, et al. Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1245−1249 doi: 10.19303/j.issn.1008-0384.2022.010.001

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Molecular Mechanism of traf6 like Gene in Innate Immunity of Procambarus clarkii

doi: 10.19303/j.issn.1008-0384.2022.010.001

1.
College of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia　014010, China
2.
Library, Inner Mongolia University of Science and Technology, Baotou, Inner Mongolia　014010, China

Received Date: 2022-01-21
Rev Recd Date: 2022-04-02
Publish Date: 2022-10-30

Abstract

Abstract

Objective Role of traf6 like gene in innate immunity regulation of Procambarus clarkia(Pc-traf6 like) was investigated. Methods RNAi technology was employed to interfere the expression of Pc-traf6 like gene. Bacterial simulation was performed to determine the changes of apoptosis-related gene expression by real-time quantitative PCR (qRT-PCR). Result The interference on the gene was effective after 48 h for the subsequent experiments. The interference-induced pro-apoptotic gene Bax, anti-apoptotic gene Bcl 2, and apoptotic protease Caspase 3 were upregulated in varying degrees with the stimulation of Staphylococcus aureus or Edwardsiella ictaluri. Conclusion After the Pc-traf6 like gene was effectively interfered, the relative expressions of Bax, Bcl 2, and Caspase 3 were upregulated after the P. clarkia was stimulated by either S. aureus or E. ictaluri indicating an involvement of Pc-traf6 like gene in the innate immune response by the regulating apoptosis.
- Procambarus clarkii,
- RNA interference,
- apoptosis,
- innate immunity

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References(21)

References

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