Effects of Substituting Refined Forage with Beer Lees on Digestion and Microbial Diversity of Hu Sheep Rumen

WANG Yinggang; ZHANG Fu; WU Xianfeng; LIN Yunqin; LI Wenyang; LIU Yuan; LIU Qinghua

doi:10.19303/j.issn.1008-0384.2023.03.002

Volume 38 Issue 3

Mar. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(3): 262-270

WANG Y G, ZHANG F, WU X F, et al. Effects of Substituting Refined Forage with Beer Lees on Digestion and Microbial Diversity of Hu Sheep Rumen [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：262−270 doi: 10.19303/j.issn.1008-0384.2023.03.002

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WANG Y G, ZHANG F, WU X F, et al. Effects of Substituting Refined Forage with Beer Lees on Digestion and Microbial Diversity of Hu Sheep Rumen [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：262−270 doi: 10.19303/j.issn.1008-0384.2023.03.002

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Effects of Substituting Refined Forage with Beer Lees on Digestion and Microbial Diversity of Hu Sheep Rumen

doi: 10.19303/j.issn.1008-0384.2023.03.002

WANG Yinggang^1
,,
ZHANG Fu^{1, 2},
WU Xianfeng^{2, 3},
LIN Yunqin⁴,
LI Wenyang^{2, 3},
LIU Yuan^{2, 3
,
,},
LIU Qinghua^{1
,
,}

1.
College of Animal Science (College of Bee science), Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Institute of Animal Husbandry and Veterinary, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
3.
Fujian Key Laboratory of Animal Genetics and Breeding, Fuzhou, Fujian　350013, China
4.
Fuqing Animal Husbandry and Veterinary Technical Service Center, Fuqing, Fujian　350300, China

Received Date: 2022-11-20
Rev Recd Date: 2023-02-01

Available Online: 2023-03-28

Publish Date: 2023-03-28

Abstract

Abstract

Objective Effects of substituting refined meal in forage with beer brewing residues on the digestion and microbial diversity of rumen of Hu sheep were studied to explore the feasibility of utilization of the waste material. Methods Twenty-four healthy Hu sheep with similar body weight of (15.90±0.36) kg were randomly divided into 4 groups. The animals were fed with a basic forage that consisted of corn and soybean meal at 36% as control (Group I) or one that used beer lees to replace 1/3 (Group II), 2/3 (Group III), or total (Group IV) of the meal. At end of the feeding period, 3 sheep were randomly selected from each group to determine the digestion indicators and microbial diversity in the rumen fluid. Results (1) None of the substitutions showed significant differences on volatile fatty acids, pH, ammonia nitrogen, or acetic acid in the rumen (P＞0.05), but significantly higher on isobutyric acid in Group II than others (P＜0.05). (2) At phylum level, no significant differences on richness of diversity were introduced by the substitutions on Firmicutes, Bacteroidetes, Proteobacteria, Synergistetes, Fibrobacteres, Actinobacteria, Epsilonbacteraeota, Spirochaetes, and Tenericutes (P＞0.05). However, the diversity of Verrucomicrobia in Group I was significantly lower (P＜0.05). At genus level, the beer lees replacements in the forage did not significantly alter the diversity on Prevotella, Succiniclasticum, Intestinimonas, Tyzzerella, Ruminococcus, Faecalicatena, Pseudobutyrivibrio, and Hungateiclostridiumhad (P＞0.05). Conclusion The replacement of the refined meal in forage with beer lees reduced the relative richness of Verrucobacteria but did not significantly changed the microbial community structure and diversity of the rumen fluid.
- Beer lees,
- Hu sheep,
- volatile fatty acids,
- rumen microorganism

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References(47)

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