Active Ingredients and Volatiles in <i>Anoectochilus roxburghii</i> Strains at Various Growth Stages

HE Hui; LIU Hongru; QIAO Yongjin; ZHANG Yi; WANG Chunfang; CHEN Bingjie; LIU Chenxia; WANG Xiao; WU Songheng

doi:10.19303/j.issn.1008-0384.2023.03.003

Volume 38 Issue 3

Mar. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(3): 271-280

HE H, LIU H R, QIAO Y J, et al. Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003

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HE H, LIU H R, QIAO Y J, et al. Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：271−280 doi: 10.19303/j.issn.1008-0384.2023.03.003

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Active Ingredients and Volatiles in Anoectochilus roxburghii Strains at Various Growth Stages

doi: 10.19303/j.issn.1008-0384.2023.03.003

HE Hui^1
,,
LIU Hongru^{1, 2},
QIAO Yongjin^{1, 2
,
,},
ZHANG Yi^{1, 2},
WANG Chunfang^{1, 2},
CHEN Bingjie^{1, 2},
LIU Chenxia^{1, 2},
WANG Xiao^{1, 2},
WU Songheng^{1, 2}

1.
Research Center of Agricultural Products Preservation and Processing, Shanghai Academy of Agricultural Sciences, Shanghai 201406, China
2.
Agri-food Storage and Processing Research Center, Shanghai Academy of Agriculture Sciences, Shanghai　201403, China

Received Date: 2022-12-27
Rev Recd Date: 2023-02-24

Available Online: 2023-03-28

Publish Date: 2023-03-28

Abstract

Abstract

Objective Contents of active ingredients and volatiles in Anoectochilus roxburghii of different strains at different growth stages were determined. Method SPME-GC-MS analysis was conducted to determine the chemical compositions in Fujian small-leaf Tuhaojin and large-leaf Dayuanbao A. roxburghii. Result The highest total polyphenols of 265.07 mg·kg⁻¹ was found in Dayuanbao 18 months after planting, so were the total flavonoids of 32.68 mg·g⁻¹ and soluble sugars of 27.71 mg·kg⁻¹ in 12-month-old and soluble protein of 600.62 mg·g⁻¹ in 18-month-old Dayunbao plants. There were 6 alcohols, 8 aldehydes, and 4 ketones and 2-pentyl-furan identified with the greatest total amount in Dayunabao grown for 24 months. The volatile content of the 6-month-old tissue culture in Tuhaojin was merely 22.9% of the peak value in the respective cultivated seedlings, and in Dayuanbao, 16.7%. The odor activity value (OAV) and principal component analysis (PCA) concluded that (E)-2-hexenal, octanal, and nonanal were the major aromatic compounds in Tuhaojin, while 1-octen-3-ol, 3,7-dimethyl-1,6-octadien-3-ol, and β-ionone in Dayuanbao. The PCA and PLS-DA indicated that these volatiles could adequately distinguish A. roxburghii of different strains harvested at different times. Conclusion Active ingredients and volatiles in A. roxburghii significantly varied by the strains and growth stages. It was plausible that a chemical analysis could suffice for quality control and authentication purpose on the products in commerce.
- Anoectochilus roxburghi,
- growth stages,
- active ingredients,
- volatile compounds

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References(31)

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