Thermal Protection of Encapsulated <i>Bacillus mucilaginosus</i>

CHEN Zhengrong; CHEN Longzhao; CHENG Yangjian; ZHANG Bingya; ZHAO Sirui; CHEN Jianmin; CHEN Yonghong; CHEN Xiaochen; LIN Rihui

doi:10.19303/j.issn.1008-0384.2023.03.013

Volume 38 Issue 3

Mar. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(3): 360-366

CHEN Z R, CHEN L Z, CHENG Y J, et al. Thermal Protection of Encapsulated Bacillus mucilaginosus [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：360−366 doi: 10.19303/j.issn.1008-0384.2023.03.013

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CHEN Z R, CHEN L Z, CHENG Y J, et al. Thermal Protection of Encapsulated Bacillus mucilaginosus [J]. Fujian Journal of Agricultural Sciences，2023，38（3）：360−366 doi: 10.19303/j.issn.1008-0384.2023.03.013

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Thermal Protection of Encapsulated Bacillus mucilaginosus

doi: 10.19303/j.issn.1008-0384.2023.03.013

1.
School of Advanced Manufacturing, Fuzhou University, Jinjiang, Fujian　362200, China
2.
Second Geological Exploration Institute, China Metallurgical Geology Bureau, Fuzhou, Fujian　350108, China
3.
Youxi Farmland Construction and Soil Fertilizer Technology Promotion Station, Youxi, Fujian　365100, China
4.
Xianyou County Agriculture and Rural Bureau, Xianyou, Fujian　351200, China
5.
College of Environment & Safety Engineering, Fuzhou University, Fuzhou, Fujian　 350108, China
6.
Zhangping Agriculture and Rural Bureau, Zhangping, Fujian　364400, China

Received Date: 2022-12-01
Accepted Date: 2022-12-01
Rev Recd Date: 2022-12-28

Available Online: 2023-03-28

Publish Date: 2023-03-28

Abstract

Abstract

Objective Effect of encapsulation to protect Bacillus mucilaginosus from thermal shock was evaluated for formulation optimization. Method Rates of survival and activation of B. mucilaginosus encapsulated with trehalose, skimmed milk powder, and/or modified H-MS medical stone after spray-drying were determined. Optimal formulation for the encapsulation was obtained using the response surface method. Result The survival rate of the naked B. mucilaginosus was (34.15±1.07)%. In contrast, the encapsulation with trehalose significantly improved the rate to (52.17±1.08)% (P＜0.01), while with H-MS, (51.64±0.77)% (P＜0.01) and with skimmed milk powder, (43.67±1.62)% (P＜0.05). Furthermore, a combined use of trehalose at 4.33%, skimmed milk powder at 2.90%, and H-MS at 7.57% for the encapsulation, the survival reached (73.32±0.76)% with a microbial count of 1.12×10⁹ CFU·g⁻¹. Conclusion The three encapsulation materials, especially, when they were applied in combination, significantly raised the B. mucilaginosus survival rate under a heat treatment. The scanning electron microscopic image revealed that trehalose and skimmed milk powder immobilized the bacteria cells in H-MS pores producing an effective thermal insulation that sheltered the encapsulated microbes.
- Medical stone,
- Bacillus mucilaginosus,
- response surface optimization,
- spray-drying,
- anti-thermal protectants

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References(28)

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