Certifying Heredity Purity of Monogerm Cytoplasmic Male Sterile and Maintainer Sugar Beet

ZHAO Yaru; PI Zhi; KAN Wenliang; WU Zedong

doi:10.19303/j.issn.1008-0384.2023.04.004

Volume 38 Issue 4

Apr. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(4): 417-422

ZHAO Y R, PI Z, KAN W L, et al. Certifying Heredity Purity of Monogerm Cytoplasmic Male Sterile and Maintainer Sugar Beet [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：417−422 doi: 10.19303/j.issn.1008-0384.2023.04.004

Citation:

ZHAO Y R, PI Z, KAN W L, et al. Certifying Heredity Purity of Monogerm Cytoplasmic Male Sterile and Maintainer Sugar Beet [J]. Fujian Journal of Agricultural Sciences，2023，38（4）：417−422 doi: 10.19303/j.issn.1008-0384.2023.04.004

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Certifying Heredity Purity of Monogerm Cytoplasmic Male Sterile and Maintainer Sugar Beet

doi: 10.19303/j.issn.1008-0384.2023.04.004

ZHAO Yaru^{1, 2
,},
PI Zhi^{1, 2},
KAN Wenliang³,
WU Zedong^{1, 2
,
,}

1.
College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, Heilongjiang　150080, China
2.
Key Laboratory of Sugar Beet Genetic Breeding(Heilongjiang University), Harbin, Heilongjiang　150080, China
3.
Heilongjiang Academy of Land Reclamation Sciences, Harbin, Heilongjiang　150080, China

Received Date: 2022-02-24
Rev Recd Date: 2022-08-04

Available Online: 2023-04-14

Publish Date: 2023-04-28

Abstract

Abstract

Objective To improve the fertility purity of beet monogerm cytoplasmic male sterile lines and maintainers. Method Forty pairs of molecular markers from a collection of 620 monogerm cytoplasmic male sterile and maintainer sugar beet germplasms were identified.All genetically mixed markers in the sterile and maintainer plants were given specific codes and recorded.Subsequently, a morphological observation was carried out in the field to exclude genetically impure plants. Result The verification of heredity purity by molecular markers revealed 92 DNA including 73 in the maintainers mixed with that of the sterile plant and 19 in the sterile lines contaminated with that belonged to the maintainer.The field examination on pollens and grains identified 122 plants (as compared to 92 isolated by the molecular marker verification) with 73 maintainers and 49 sterile lines to be promiscuous.In addition, polygerm plants were unexpectedly found in the lot that could have grown from the accidentally included seeds. Conclusion By excluding adulterated plants in the field, the purity on heredity of sterile and maintainer lines could be assured.The procedure would provide the prerequisite essential for accurate genetic diversity determination and successful preparation of binary sterile sugar beet lines.
- Sugar beet,
- molecular marker,
- heredity certification,
- morphology,
- screening

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References(21)

References

[1]	IVANA V, ZDENKO L, SUZANA K, et al. Sugar beet root yield and quality with leaf seasonal dynamics in relation to planting densities and nitrogen fertilization [J]. Agriculture, 2021, 11(5): 407. doi: 10.3390/agriculture11050407
[2]	苏欣欣, 胡晓航, 马亚怀, 等. 滤泥施用量对不同品种甜菜产量及土壤中氮磷钾含量的影响 [J]. 中国农学通报, 2022, 38(11):38−45. doi: 10.11924/j.issn.1000-6850.casb2021-0661 SU X X, HU X H, MA Y H, et al. Effect of sugar mill filter mud application rate on yield of sugar beet varieties and contents of nitrogen, phosphorus and potassium in soil [J]. Chinese Agricultural Science Bulletin, 2022, 38(11): 38−45.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb2021-0661
[3]	EBMEYER H , FIEDLER-WIECHERS K , HOFFMANN C M. Drought tolerance of sugar beet - Evaluation of genotypic differences in yield potential and yield stability under varying environmental conditions [J]. European Journal of Agronomy, 2021, 125: 126262. doi: 10.1016/j.eja.2021.126262
[4]	OWEN F V. The sugar beet breeder's problem of establishing male-sterile populations for hybridization purposes [J]. Proc Am Soc Sugar Beet Technol., 1950, 6: 191−194.
[5]	冯小磊, 范光宇, 苏旭, 等. 植物雄性不育生理生化研究进展 [J]. 作物杂志, 2012(3):6−11. doi: 10.16035/j.issn.1001-7283.2012.03.005 FENG X L, FAN G Y, SU X, et al. Advances in physiological and biochemical study on plant male sterility [J]. Crops, 2012(3): 6−11.(in Chinese) doi: 10.16035/j.issn.1001-7283.2012.03.005
[6]	王昱丹. 甜瓜雄性不育苗期早期鉴定方法[D]. 大庆: 黑龙江八一农垦大学, 2018 WANG Y D. Early identification method of male sterility in muskmelon seedling stage[D]. Daqing: Heilongjiang Bayi Agricultural University, 2018. (in Chinese)
[7]	柴军琳, 孙阳阳, 贾小平, 等. 一种普通小麦细胞质雄性不育系的鉴定及恢保关系研究 [J]. 麦类作物学报, 2016, 36(8):1003−1007. doi: 10.7606/j.issn.1009-1041.2016.08.05 CHAI J L, SUN Y Y, JIA X P, et al. Identification and restoring-maintaining relationship of a cytoplasmic male sterile line in common wheat [J]. Journal of Triticeae Crops, 2016, 36(8): 1003−1007.(in Chinese) doi: 10.7606/j.issn.1009-1041.2016.08.05
[8]	刘一珺. 甜菜Owen型育性分子鉴定及分子辅助育种研究[D]. 哈尔滨: 哈尔滨工业大学, 2015 LIU Y J. Molecular identification and molecular-assisted breeding of Owen type fertility in sugarbeet[D]. Harbin: Harbin Institute of Technology, 2015. (in Chinese)
[9]	NISHIZAWA S, KUBO T, MIKAMI T. Variable number of tandem repeat loci in the mitochondrial genomes of beets [J]. Current Genetics, 2000, 37(1): 34−38. doi: 10.1007/s002940050005
[10]	刘巧红, 程大友, 罗成飞, 等. 甜菜TR1位点多态性分析及细胞质育性鉴定 [J]. 中国甜菜糖业, 2013(1):18−20,24. doi: 10.3969/j.issn.1002-0551.2013.01.005 LIU Q H, CHENG D Y, LUO C F, et al. TR1 allele polymorphism analysis and cytoplasm fertility identification for 8 sugar beet material populations [J]. China Beet & Sugar, 2013(1): 18−20,24.(in Chinese) doi: 10.3969/j.issn.1002-0551.2013.01.005
[11]	唐文帮, 肖应辉, 王建龙, 等. 水稻光温敏核不育系株系育性鉴定保纯法原种生产技术 [J]. 杂交水稻, 2010, 25(5):25−27,33. doi: 10.3969/j.issn.1005-3956.2010.05.009 TANG W B, XIAO Y H, WANG J L, et al. A foundation seed production method to ensure seed purity of rice PTGMS lines by fertility identification of plant lines [J]. Hybrid Rice, 2010, 25(5): 25−27,33.(in Chinese) doi: 10.3969/j.issn.1005-3956.2010.05.009
[12]	张井勇. 大豆细胞质雄性不育“三系”异交率相关性状及其异交率鉴定方法的研究[D]. 哈尔滨: 东北农业大学, 2018. ZHANG J Y. Study on outcrossing rate related characters of soybean cytoplasmic male sterility “three lines” and its identification method[D]. Harbin: Northeast Agricultural University, 2018. (in Chinese)
[13]	杨金华, 杜黎君, 贾菲芸, 等. 基于冬季负积温预测小麦BNS雄性不育系育性的转换率 [J]. 甘肃农业大学学报, 2021, 56(4):31−35,42. doi: 10.13432/j.cnki.jgsau.2021.04.005 YANG J H, DU L J, JIA F Y, et al. Prediction of fertility conversion rate of wheat BNS male sterile lines based on negative accumulated temperature in winter [J]. Journal of Gansu Agricultural University, 2021, 56(4): 31−35,42.(in Chinese) doi: 10.13432/j.cnki.jgsau.2021.04.005
[14]	BLUMWALD E, POOLE R J. Salt tolerance in suspension cultures of sugar beet: Induction of na/h antiport activity at the tonoplast by growth in salt [J]. Plant Physiology, 1987, 83(4): 884−887. doi: 10.1104/pp.83.4.884
[15]	HAJHEIDARI M, ABDOLLAHIAN-NOGHABI M, ASKARI H, et al. Proteome analysis of sugar beet leaves under drought stress [J]. Proteomics, 2005, 5(4): 950−960. doi: 10.1002/pmic.200401101
[16]	KLEUKER G, HOFFMANN C M. Causes of different tissue strength, changes during storage and effect on the storability of sugar beet genotypes [J]. Postharvest Biology and Technology, 2022, 183: 111744. doi: 10.1016/j.postharvbio.2021.111744
[17]	王良, 白晨, 李晓东, 等. VNTR分子标记技术在甜菜不育系选育中的应用 [J]. 华北农学报, 2014, 29(4):135−139. doi: 10.7668/hbnxb.2014.04.023 WANG L, BAI C, LI X D, et al. VNTR molecular marker technology in the application of beet sterile lines breeding [J]. Acta Agriculturae Boreali-Sinica, 2014, 29(4): 135−139.(in Chinese) doi: 10.7668/hbnxb.2014.04.023
[18]	MORITANI M, TAGUCHI K, KITAZAKI K, et al. Identification of the predominant nonrestoring allele for Owen-type cytoplasmic male sterility in sugar beet (Beta vulgaris L. ): Development of molecular markers for the maintainer genotype [J]. Molecular Breeding, 2013, 32(1): 91−100. doi: 10.1007/s11032-013-9854-8
[19]	石好琪, 吴则东, 兴旺, 等. 甜菜多胚种质资源育性组成的分子标记分析 [J]. 中国糖料, 2021, 43(4):26−31. doi: 10.13570/j.cnki.scc.2021.04.004 SHI H Q, WU Z D, XING W, et al. Molecular marker analysis of fertility composition of sugarbeet multi-embryo germplasm resources [J]. Sugar Crops of China, 2021, 43(4): 26−31.(in Chinese) doi: 10.13570/j.cnki.scc.2021.04.004
[20]	石好琪, 丁刘慧子, 邳植, 等. 利用分子标记快速鉴定甜菜育性的研究 [J]. 中国农学通报, 2021, 37(3):61−65. doi: 10.11924/j.issn.1000-6850.casb2020-0446 SHI H Q, DING L H Z, PI Z, et al. Study on rapid identification of sugarbeet fertility by molecular markers [J]. Chinese Agricultural Science Bulletin, 2021, 37(3): 61−65.(in Chinese) doi: 10.11924/j.issn.1000-6850.casb2020-0446
[21]	孟祥雯, 程大友, 崔杰, 等. 甜菜Owen型质核互作雄性不育系及保持系花蕾cDNA-AFLP多态性分析 [J]. 分子植物育种, 2016, 14(1):135−140. doi: 10.13271/j.mpb.014.000135 MENG X W, CHENG D Y, CUI J, et al. Bud of sugar beet c DNA-AFLP polymorphism analysis of Owen-type male sterile line and maintainer line [J]. Molecular Plant Breeding, 2016, 14(1): 135−140.(in Chinese) doi: 10.13271/j.mpb.014.000135