Pollen Cytology, Stamen Callus Induction, and Seedling Propagation of <i>Paeonia delavayi</i>

MOU Yaping; ZHANG Pingfang; DU Chun; LI Jin; LIU Zirong; WANG Juan

doi:10.19303/j.issn.1008-0384.2023.09.008

Volume 38 Issue 9

Sep. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(9): 1064-1072

MOU Y P, ZHANG P F, DU C, et al. Pollen Cytology, Stamen Callus Induction, and Seedling Propagation of Paeonia delavayi [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1064−1072 doi: 10.19303/j.issn.1008-0384.2023.09.008

Citation:

MOU Y P, ZHANG P F, DU C, et al. Pollen Cytology, Stamen Callus Induction, and Seedling Propagation of Paeonia delavayi [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1064−1072 doi: 10.19303/j.issn.1008-0384.2023.09.008

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Pollen Cytology, Stamen Callus Induction, and Seedling Propagation of Paeonia delavayi

doi: 10.19303/j.issn.1008-0384.2023.09.008

MOU Yaping^{1, 2
,},
ZHANG Pingfang¹,
DU Chun³,
LI Jin¹,
LIU Zirong¹,
WANG Juan^{4
,
,}

1.
College of Landscape Architecture, Southwest Forestry University, Kunming, Yunnan　650224, China
2.
Key Laboratory of Biodiversity Conservation in Southwest China, National Forestry and Grassland Bureau, Kunming, Yunnan　650224, China
3.
Forestry College, Southwest Forestry University, Kunming, Yunnan　650224, China
4.
Green Development Research Institute, Southwest Forestry University, Kunming, Yunnan　650224, China

Received Date: 2023-05-10
Rev Recd Date: 2023-07-16

Available Online: 2023-09-19

Publish Date: 2023-09-28

Abstract

Abstract

Objective Developments of flower buds and pollen grains, optimum medium for stamen callus induction, and proper conditions for seedling propagation and differentiation of Paeonia delavayi were investigated. Method Dominant pollen grain development stages were examined under an optical microscope on the paraffin sections of red P. delavayi hard buds with visible color. On an MS solid medium, various plant growth regulators in different concentrations were added to determine the optimal formulation for the induction, proliferation, and differentiation of stamen callus. Result (1) The prevailing stage of pollen grain development for the redP. delavayi, Y2-Y4, occurred in the mid-mononuclear period when the bud diameter was 10-17 mm but the color had yet to show. At T1-T2, the bud grew to a diameter ranging from 12 mm to 15 mm with less than 30% of the bud showing its color. That was in the single-core sideline period. When the diameter of a stamen reached 15-19 mm with a colored area greater than 30%, the T3-T4 stage was in the binuclear period. (2) The stamens were most effectively disinfected with running water for 30min followed by a rinse with 1% sodium hypochlorite for 8min. (3) The stamen could be optimally induced during the single-core sideline period. (4) The medium for optimal stamen callus induction was formulated with MS + 0.2 mg NAA·L⁻¹ + 0.5 g PVP·L⁻¹, and that for the proliferation MS + 0.5 mg NAA·L⁻¹ + 0.25 mg 6-BA·L⁻¹ + 0.5 mg GA₃·L⁻¹ + 1 g PVP·L⁻¹. Conclusion This study clearly revealed the floral bud size, appearance, and dominant pollen grain development stages of the red P. delavayi and determined the optimal medium formulations and culture conditions for inducing, proliferating, and differentiating the stamen callus.
- Paeonia delavayi,
- stamens,
- callus,
- plant propagation

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References(38)

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