SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood

LIU Wenjing; PAN Wei; LIN Huizhen; CHEN Hongju; YAN Shaode

doi:10.19303/j.issn.1008-0384.2024.03.014

Volume 39 Issue 3

Mar. 2024

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(3): 362-368

LIU W J, PAN W, LIN H Z, et al. SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：362−368 doi: 10.19303/j.issn.1008-0384.2024.03.014

Citation:

LIU W J, PAN W, LIN H Z, et al. SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood [J]. Fujian Journal of Agricultural Sciences，2024，39（3）：362−368 doi: 10.19303/j.issn.1008-0384.2024.03.014

Citation:

PDF( 1085 KB)

SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood

doi: 10.19303/j.issn.1008-0384.2024.03.014

1.
Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Quality & Safety, Fuzhou, Fujian　350003, China
2.
Xiamen PERSer Nanotechnology Co., Ltd., Xiamen, Fujian　361021, China
3.
Xiamen Assessment Service Center of Quality and Technology, Xiamen, Fujian　361021, China

Received Date: 2023-06-06
Rev Recd Date: 2023-12-07

Available Online: 2024-03-28

Publish Date: 2024-03-28

Abstract

Abstract

Objective A rapid method for detecting residues of enrofloxacin (ENR) and ciprofloxacin (CIP) in seafood by surface-enhanced Raman spectroscopy (SERS) was developed. Method On spiked seafood samples, (50±5) nm silver nanoparticles were used as the enhancement reagent and 200 g·L⁻¹ NaCl solution as the extraction solution for the SERS assay. Detection results were read within 3 min and mathematical correlation between peak measurement and drug concentration examined. Based on the linearity and limit of detection, optimal assay conditions were determined. Result The assay showed several peaks on ENR and CIP standards. The C-N bending vibration caused the peak at 532 cm⁻¹, the out-of-plane bending vibration at 552 cm⁻¹, the C-N, C-C-F out-of-plane and C-C=O in-plane bending vibrations at 651 cm⁻¹, the stretching vibration of C-H on the benzene ring, C-C=O out-of-plane bending vibration, C-C-N bending vibration and C-H, C-N stretching vibration at 737 cm⁻¹, and the C-H, C-N out-of-plane bending vibration at 785 cm⁻¹. By quantifying the characteristic peak at 737 cm⁻¹, a linear relationship with an R²＞0.96 was found between the Raman intensity and drug concentration in the range of 10–200 ng·mL⁻¹. The assay had a recovery rate of 78.4%~106.7%, RSD of 2.1%~6.7%, and a detection limit of 10 μg·kg⁻¹. Conclusion The newly developed rapid SERS assay for detecting ENR and CIP residues in seafood was deemed accurate, stable, and applicable for field screening and testing.
- Surface-enhanced Raman spectroscopy,
- enrofloxacin,
- ciprofloxacin,
- rapid detection

FullText(HTML)

References(28)

References

[1]	LÓPEZ-CADENAS C, SIERRA-VEGA M, GARCÍA-VIEITEZ J J, et al. Enrofloxacin: Pharmacokinetics and metabolism in domestic animal species [J]. Current Drug Metabolism, 2013, 14(10): 1042−1058. doi: 10.2174/1389200214666131118234935
[2]	罗贤静丽. 超高效液相色谱-串联质谱法测定鱼肉组织中的恩诺沙星、环丙沙星和沙拉沙星残留量 [J]. 现代食品, 2020, (7):170−171,179. LUO X J L. Determination of enrofloxacin, ciprofloxacin and sarafloxacin in fish tissue by UPLC-MS/MS [J]. Modern Food, 2020(7): 170−171,179. (in Chinese)
[3]	BADAWY S, YANG Y Q, LIU Y N, et al. Toxicity induced by ciprofloxacin and enrofloxacin: Oxidative stress and metabolism [J]. Critical Reviews in Toxicology, 2021, 51(9): 754−787. doi: 10.1080/10408444.2021.2024496
[4]	MA R R, HUANG L, WEI W J, et al. Pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin in Pacific white shrimp Litopenaeus vannamei after multiple-dose oral administration [J]. Fisheries Science, 2018, 84(5): 869−876. doi: 10.1007/s12562-018-1229-y
[5]	李倩, 王甲, 张玉洁, 等. 动物性食品中喹诺酮类药物残留检测方法研究进展 [J]. 食品安全质量检测学报, 2021, 12(8):3016−3022. LI Q, WANG J, ZHANG Y J, et al. Research progress on determination methods of quinolone residues in animal food [J]. Journal of Food Safety & Quality, 2021, 12(8): 3016−3022. (in Chinese)
[6]	TROUCHON T, LEFEBVRE S. A review of enrofloxacin for veterinary use [J]. Open Journal of Veterinary Medicine, 2016, 6(2): 40−58. doi: 10.4236/ojvm.2016.62006
[7]	张秀媛, 何扩, 黄智鸿, 等. 恩诺沙星抗体免疫检测及其分子识别机制研究 [J]. 现代食品科技, 2015, 31(6):284−289. ZHANG X Y, HE K, HUANG Z H, et al. Immunodetection and molecular recognition mechanism of antibodies against enrofloxacin [J]. Modern Food Science and Technology, 2015, 31(6): 284−289. (in Chinese)
[8]	李继昌, 鲁成武, 符春燕, 等. 氟喹诺酮类药物在禽病防治上的应用 [J]. 黑龙江畜牧兽医, 1998, (10):27. LI J C, LU C W, FU C Y, et al. Application of fluoroquinolones in prevention and treatment of poultry diseases [J]. Heilongjiang Animal Science and Veterinary Medicine, 1998(10): 27. (in Chinese)
[9]	梁惜梅, 施震, 黄小平. 珠江口典型水产养殖区抗生素的污染特征 [J]. 生态环境学报, 2013, 22(2):304−310. doi: 10.3969/j.issn.1674-5906.2013.02.022 LIANG X M, SHI Z, HUANG X P. Occurrence of antibiotics in typical aquaculture of the Pearl River Estuary [J]. Ecology and Environmental Sciences, 2013, 22(2): 304−310. (in Chinese) doi: 10.3969/j.issn.1674-5906.2013.02.022
[10]	DALLA BONA M, ZOUNKOVÁ R, MERLANTI R, et al. Effects of enrofloxacin, ciprofloxacin, and trimethoprim on two generations of Daphnia magna [J]. Ecotoxicology and Environmental Safety, 2015, 113: 152−158. doi: 10.1016/j.ecoenv.2014.11.018
[11]	中华人民共和国农业农村部. 食品安全国家标准食品中兽药最大残留限量GB 31650-2019[S]. 北京: 中国农业出版社, 2020.
[12]	CHAKRAVARTHY V A, SAILAJA B B V, KUMAR A P. Stability-indicating RP-HPLC method for simultaneous estimation of enrofloxacin and its degradation products in tablet dosage forms [J]. Journal of Analytical Methods in Chemistry, 2015, 2015: 735145.
[13]	TIAN H Z. Determination of chloramphenicol, enrofloxacin and 29 pesticides residues in bovine milk by liquid chromatography-tandem mass spectrometry [J]. Chemosphere, 2011, 83(3): 349−355. doi: 10.1016/j.chemosphere.2010.12.016
[14]	苏晓晴, 张李琦, 郑明学, 等. 紫外分光光度法测定恩诺沙星微囊含量方法的建立 [J]. 山西农业大学学报(自然科学版), 2017, 37(4):258−262. SU X Q, ZHANG L Q, ZHENG M X, et al. Determination of enrofloxacin gelatin microcapsules content by UV spectrophotometry [J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2017, 37(4): 258−262. (in Chinese)
[15]	LEI X L, XU X X, LIU L Q, et al. Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat [J]. Journal of Dairy Science, 2022, 105(3): 1999−2010. doi: 10.3168/jds.2021-20276
[16]	杨熠, 孟坤杰, 赵红琼, 等. 酶联免疫吸附法检测动物源性食品中恩诺沙星残留量 [J]. 食品科学, 2017, 38(8):239−243. YANG Y, MENG K J, ZHAO H Q, et al. ELISA determination of enrofloxacin [J]. Food Science, 2017, 38(8): 239−243. (in Chinese)
[17]	PANZENHAGEN P H N, AGUIAR W S, GOUVÊA R, et al. Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS[J]. Food Additives & Contaminants Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 2016, 33(4): 639-643.
[18]	ZHANG Z Z, LIU Q, ZHANG M, et al. Simultaneous detection of enrofloxacin and ciprofloxacin in milk using a bias potentials controlling-based photoelectrochemical aptasensor [J]. Journal of Hazardous Materials, 2021, 416: 125988. doi: 10.1016/j.jhazmat.2021.125988
[19]	NILGHAZ A, MAHDI MOUSAVI S, AMIRI A, et al. Surface-enhanced Raman spectroscopy substrates for food safety and quality analysis [J]. Journal of Agricultural and Food Chemistry, 2022, 70(18): 5463−5476. doi: 10.1021/acs.jafc.2c00089
[20]	李晨, 赵超敏, 古淑青, 等. 水产品中孔雀石绿和结晶紫残留的拉曼光谱法快速检测 [J]. 现代食品科技, 2022, 38(3):286−292,298. LI C, ZHAO C M, GU S Q, et al. Rapid determination of malachite green and crystal violet residues in aquatic products by Raman spectroscopy [J]. Modern Food Science and Technology, 2022, 38(3): 286−292,298. (in Chinese)
[21]	马海宽, 韩晓红, 张财华, 等. 鱼肉中磺胺类抗生素的表面增强拉曼光谱探测与分析 [J]. 激光生物学报, 2014, 23(6):560−565. doi: 10.3969/j.issn.1007-7146.2014.06.009 MA H K, HAN X H, ZHANG C H, et al. The study of sulfonamide antibiotics in fish based on surface-enhanced Raman spectroscopy technology [J]. Acta Laser Biology Sinica, 2014, 23(6): 560−565. (in Chinese) doi: 10.3969/j.issn.1007-7146.2014.06.009
[22]	李静, 余婉松, 夏苏捷, 等. 基于表面增强拉曼光谱的养殖水中硝基呋喃类抗生素残留检测 [J]. 食品工业科技, 2019, 40(24):225−230,236. LI J, YU W S, XIA S J, et al. Detection of nitrofuran antibiotics residues in fishery water by surface-enhanced Raman spectroscopy [J]. Science and Technology of Food Industry, 2019, 40(24): 225−230,236. (in Chinese)
[23]	宋洪艳, 赵航, 严霞, 等. 基于表面增强拉曼光谱技术的海洋污染物多氯联苯吸附特性分析 [J]. 光谱学与光谱分析, 2022, 42(3):704−712. SONG H Y, ZHAO H, YAN X, et al. Adsorption characteristics of marine contaminant polychlorinated biphenyls based on surface-enhanced Raman spectroscopy [J]. Spectroscopy and Spectral Analysis, 2022, 42(3): 704−712. (in Chinese)
[24]	孙琳, 张涵, 杜一平. 基于SBA-15的表面增强拉曼基底的制备及对鸡肉和鸡饲料中恩诺沙星的检测 [J]. 高等学校化学学报, 2018, 39(3):455−462. doi: 10.7503/cjcu20170328 SUN L, ZHANG H, DU Y P. Preparation of surface enhanced Raman scattering substrates based on SBA-15 material and the detection of enrofloxacin in chicken and chicken feed [J]. Chemical Journal of Chinese Universities, 2018, 39(3): 455−462. (in Chinese) doi: 10.7503/cjcu20170328
[25]	FRENS G. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions [J]. Nature Physical Science, 1973, 241(105): 20−22. doi: 10.1038/physci241020a0
[26]	YU W W, WHITE I M. A simple filter-based approach to surface enhanced Raman spectroscopy for trace chemical detection [J]. The Analyst, 2012, 137(5): 1168−1173. doi: 10.1039/c2an15947c
[27]	朱圣清, 杨芳. 基于Drude-Lorentz模型的金银纳米颗粒光谱特征研究 [J]. 江苏理工学院学报, 2022, 28(4):9−16. ZHU S Q, YANG F. Research on the spectral characteristics of gold and silver nanoparticles based on Drude-Lorentz model [J]. Journal of Jiangsu University of Technology, 2022, 28(4): 9−16. (in Chinese)
[28]	郑红. 基于表面增强拉曼光谱技术的环境水样中痕量磺胺类和喹诺酮类抗生素的检测研究[D]. 厦门: 厦门大学, 2019. ZHENG H. Study on the detection of trace sulfonamides and quinolones in environment water samples by surface-enhanced Raman spectroscopy[D]. Xiamen: Xiamen University, 2019. (in Chinese)