Application of <i>Bacillus subtilis</i> B2-GFP to Promote Growth of Sweet Pepper Seedlings

ZHAO Li; SHANG Ye; YAN Gengyang; LIU Ju; ZHANG Juping

doi:10.19303/j.issn.1008-0384.2024.04.009

Volume 39 Issue 4

Apr. 2024

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > 39(4): 448-455

ZHAO L, SHANG Y, YAN G Y, et al. Application of Bacillus subtilis B2-GFP to Promote Growth of Sweet Pepper Seedlings [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：448−455 doi: 10.19303/j.issn.1008-0384.2024.04.009

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ZHAO L, SHANG Y, YAN G Y, et al. Application of Bacillus subtilis B2-GFP to Promote Growth of Sweet Pepper Seedlings [J]. Fujian Journal of Agricultural Sciences，2024，39（4）：448−455 doi: 10.19303/j.issn.1008-0384.2024.04.009

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Application of Bacillus subtilis B2-GFP to Promote Growth of Sweet Pepper Seedlings

doi: 10.19303/j.issn.1008-0384.2024.04.009

College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, Henan　471000, China

Received Date: 2024-01-15
Accepted Date: 2024-04-12
Rev Recd Date: 2024-03-19

Available Online: 2024-06-26

Publish Date: 2024-04-28

Abstract

Abstract

Objective Effects of applying Bacillus subtilis B2-GFP culture broth on the growth and physiology of sweet pepper seedlings were studied. Methods In a pot experiment, seedlings of sweet pepper Shuoyuan 808 were treated with B. subtilis B2-GFP culture broths at the concentrations of 1×10⁵CFU·mL⁻¹ (T1), 1×10⁶CFU·mL⁻¹ (T2), 1×10⁷CFU·mL⁻¹ (T3), and 1×10⁸CFU·mL⁻¹ (T4), along with non-treatment control (CK). The transplanted sweet pepper seedlings were given 5 mL 1×10⁶CFU·mL_⁻¹B2-GFP culture broth every 7 d for 3 times. Growth index, biomass accumulation, leaf photosynthesis, and chlorophyll fluorescence parameters as well as antioxidant enzyme activities, morphological formation, and root vitality of the plants were monitored at 21 d. Results Application of the B2-GFP culture broth increased the plant height and biomass as well as the leaf area and chlorophyll content over CK. The greatest effect was observed under T2, which also accentuated the leaf transpiration rate (T_r), stomatal conductance (G_s), maximum fluorescence (F_m), photosystem II potential Activity (Ф_PSⅡ), and photochemical fluorescence quenching coefficient (qP) as well as the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), phenylalanine ammonia lyase (PAL), and polyphenol oxidase (PPO) in the roots but reduced the intercellular CO₂ concentration (C_i) and chlorophyll basic fluorescence (F_o). Conclusion When the transplanted sweet pepper seedlings were given 1×10⁶CFU·mL⁻¹ B2-GFP culture broth, all monitored indicators on the plant growth and root development including leaf photosynthetic parameters and biomass and root antioxidant enzymes activities and vitality were significantly improved in 3 weeks.
- Bacillus subtilis,
- sweet pepper,
- growth,
- physiology,
- photosynthesis

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References(23)

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