Diversity of Endophytic Bacteria in Root Nodules of Different Cultivars of <i>Astragalus Sinicus</i> L

FANG Yu; LIU Cailing; LIN Chenqiang; CHEN Jichen; JIA Xianbo

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Article Navigation > Fujian Journal of Agricultural Sciences > 2024 > Accepted Manuscript

FANG Y, LIU C L, LIN C Q, et al. Diversity of Endophytic Bacteria in Root Nodules of Different Cultivars of Astragalus Sinicus L [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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FANG Y, LIU C L, LIN C Q, et al. Diversity of Endophytic Bacteria in Root Nodules of Different Cultivars of Astragalus Sinicus L [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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Diversity of Endophytic Bacteria in Root Nodules of Different Cultivars of Astragalus Sinicus L

Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

Received Date: 2024-03-14
Rev Recd Date: 2024-05-07

Available Online: 2024-07-10

Abstract

Abstract

: [ Objective ] Analyzing the community structure of endophytic bacteria in root nodules is important to develop the rhizobium inoculants and understand the mechanism of fixing nitrogen. [ Method ] This study was conducted to investigate the diversity of endophytic bacteria in root nodules of A. sinicus. Seven cultivars of A. sinicus planted in Fujian province were selected to analyze the diversity of endophytic bacteria in root nodules using 16S rRNA gene as the target gene with Illumina Miseq PE300 sequencing platform. [ Result ] C ultivar could impact the composition of nodule endophytic bacteria. The most abundant genus in ZZ5 root nodule was Pseudomonas but the predominnat genus was Mesorhizobiumin in other cultivars. Furthermore, we found high Shannon indices of endophytic bacterial communities in the root nodules of MZ8, XY and ZZ5. These cultivars had a large diversity of non-rhizobial endophytes like Pseudomonas, Stenotrophomonas, Duganella and Erwinia, etc. The higher relative abundances of pathogen Erwinia rhapontici and Pantoea ananatis PA13 were observed in the root nodules of MZ8, XY and ZZ5. [ Conclusions ] The predominant endophytic bacteria of cultivar ZZ5 is non-rhizobial microorganism Pesudomonas which had the growth-promoting effect, while rhizobia are the predominant microorganism in other cultivars. Different cultivars can be applied in combination to get more effect.
- Astragalus sinicus,
- cultivar,
- root nodule,
- endophytic bacteria,
- diversity

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References(30)

References

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