Physiological changes and gene expression patterns of <i>Polygonatum</i> <i>cyrtonema</i> Hua seeds during physiological post-ripening process

SU Hailan; NIU Yuqing; CHENG Jianhua; ZHU Yanming; CHEN Hong; ZHENG Meixia; HUANG Xiangui; ZHU Yujing

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SU H L, NIU Y Q, CHENG J H, et al. Physiological changes and gene expression patterns of Polygonatum cyrtonema Hua seeds during physiological post-ripening process [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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SU H L, NIU Y Q, CHENG J H, et al. Physiological changes and gene expression patterns of Polygonatum cyrtonema Hua seeds during physiological post-ripening process [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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Physiological changes and gene expression patterns of Polygonatum cyrtonema Hua seeds during physiological post-ripening process

1.
Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Shaowu Forest Bureau, Nanping, Fujian　354000, China
3.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350012, China

Received Date: 2024-01-11
Rev Recd Date: 2024-03-18

Available Online: 2024-06-26

Abstract

Abstract

Objective In order to study the physiological and biochemical changes and differential gene expression of Polygonatum cyrtonema Hua seeds during physiological post-ripening. Method the contents of superoxide dismutase, α-amylase, β-amylase, soluble protein, crude fat and soluble sugar at each stage were analyzed by chemical determination method. The differentially expressed genes in 6 stages were analyzed by high-throughput sequencing technology, and the expression levels of key differentially expressed genes were verified by qRT-PCR. Result (1) During the physiological post-ripening process of Polygonatum cyrtonema Hua seeds, the soluble sugar content increased gradually in the first four stages, and slightly decreased in the last two stages; The crude fat and protein showed a decreasing trend. The activity of α-amylase and β-amylase increased. (2) During physiological postripening of Polygonatum cyrtonema Hua seeds, differentially expressed genes were significantly enriched in sucrose and starch metabolic pathways and hormone signal transduction pathways. Conclusion During the physiological post-ripening process of Polygonatum cyrtonema Hua seeds, the conversion of starch and other nutrients and the coordination of endogenous hormones promoted the physiological post-ripening of Polygonatum cyrtonema Hua seeds. This study is of great significance for understanding the physiological and biochemical changes of Polygonatum cyrtonema Hua seeds during post-ripening and the molecular mechanism of dormancy release.
- Polygonatum cyrtonema Hua,
- physiological afterripening,
- transcriptome sequencing analysis

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References(28)

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