Study on liquid spawn reproductive technology of <i>Stropharia rugosoannulata</i>

ZENG Zhiheng; DAI Jianqing; CHEN Wenzhi; ZENG Hui; GUO Zhongjie; CAI Zhixin

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ZENG Z H, DAI J Q, CHEN W Z, et al. Study on liquid spawn reproductive technology of Stropharia rugosoannulata [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−10

Citation:

ZENG Z H, DAI J Q, CHEN W Z, et al. Study on liquid spawn reproductive technology of Stropharia rugosoannulata [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−10

ZENG Z H, DAI J Q, CHEN W Z, et al. Study on liquid spawn reproductive technology of Stropharia rugosoannulata [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−10

Citation:

ZENG Z H, DAI J Q, CHEN W Z, et al. Study on liquid spawn reproductive technology of Stropharia rugosoannulata [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−10

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Study on liquid spawn reproductive technology of Stropharia rugosoannulata

Edible Fungi Institute of Fujian Academy of Agricultural Sciences/National and Local Joint Engineering ResearchCenter for Breeding & Cultivation of Featured Edible Fungi, Fuzhou, Fujian　350014, China

Received Date: 2024-04-12
Rev Recd Date: 2024-06-14

Available Online: 2024-07-10

Abstract

Abstract

Objective To optimize the liquid culture medium for Stropharia rugosoannulata and investigate its growth patterns during the liquid culture process, thereby establishing the parameters for the reproduction and cultivation of the liquid spawn. Methods Using S^. rugosoannulata No.8 as the experimental strain, the mycelial biomass was taken as the evaluation index. A single factor and orthogonal test L₉(3⁴) were employed to optimize the liquid culture medium. The mycelial biomass, reducing sugars, and amino nitrogen content, as well as the extracellular enzyme activities of carboxymethyl cellulase, amylase, acid protease, and laccase of the optimized liquid culture were measured to determine the end point of liquid culture. The average full-bag time was used as the indicator to establish the inoculation amount, particle size of the substrate formulation, and carbon-to-nitrogen ratio for reproduction and cultivation of the liquid spawn. Results The optimal formulation for the liquid spawn of S. rugosoannulata was determined to be 20 g·L⁻¹ glucose, 30 g·L⁻¹ wheat flour, 0.75 g·L⁻¹ yeast powder, 1.00 g·L⁻¹ potassium dihydrogen phosphate, and 0.50 g·L⁻¹ magnesium sulfate, with an initial pH of 5. On the 8th day of culture, the mycelial biomass reached a maximum at 1.66 g/100mL. The content of reducing sugar decreased from 12.23 mg·L⁻¹ to 1.38 mg·L⁻¹, and the content of amino nitrogen decreased from 0.09 mg·L⁻¹ to 0.06 mg·L⁻¹ during the liquid culture process. The activities of carboxymethyl cellulase and amylase was the highest on the 4 th day with the activity of 6.49 U and 5.16 U, respectively. The activity of acid protease was the highest on the 2 nd day, and the enzyme activity was 1.80 U. The laccase activity was the highest on the 6 th day, and the enzyme activity was 1.63 U. The production parameters for the breeding and cultivation of the liquid inoculum were: an inoculation amount of 15 mL, a coarse-to-fine ratio of 7:3 for the particle size of the substrate formulation, and a carbon-to-nitrogen ratio of 50:1. Conclusion The viability of liquid spawn of S. rugosoannulata correlated with some of selected physiological and biochemical indices. Combining the physiological and biochemical indicators of the fermentation fluid, the highest vitality of the liquid spawn was judged to be on the 7th day. Using of liquid spawn of S. rugosoannulata for reproduction and cultivation, the average hyphal pocketful time is 23.7 d, which can shorten the growth cycle by 2.7 d. The research results have established the formulation of the liquid culture medium and the accompanying spawn-making process for S. rugosoannulata, laying the foundation for the industrial spawn production technology.
- Stropharia rugosoannulata,
- liquid spawn,
- medium,
- optimization,
- application

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References(27)

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